牙龈卟啉单胞菌脂多糖诱导组织蛋白酶D在牙周炎中的作用

IF 1.8 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Hyun Woong Jeong, Dong Sik Chang, June Soo Kim, Young Sun Hwang
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引用次数: 0

摘要

牙周炎是一种由口腔细菌引起的牙齿支持组织炎症性疾病。进行性牙周炎发生牙周韧带丢失和牙槽骨破坏。由于龈沟液(GCF)反映了牙周袋的炎症环境,因此它是开发牙周炎诊断靶点的非常重要的样本。使用从健康参与者和牙周炎患者收集的GCF进行抗体阵列,以鉴定与牙周炎有关的蛋白水解酶。在抗体阵列膜上的21个靶标中,激肽释放酶6(KLK6)、激肽释放蛋白10(KLK10)、组织蛋白酶A(CathA)和组织蛋白酶D(CathD)在牙周炎GCF中的水平高于健康参与者的GCF。在四个靶标中,脂多糖刺激永生化牙龈成纤维细胞中的牙龈卟啉单胞菌(PG-LPS)仅增加CathD蛋白水平。在PG-LPS处理的永生化牙龈成纤维细胞提取物中,CathD的底物切割活性增加。PG-LPS诱导的底物切割作用被CathD抑制剂pepstatin A消除。用PG-LPS处理的永生化牙龈成纤维细胞的条件培养基处理可促进破骨细胞的形成,但被CathD抑制物pepstin A抑制。这些结果表明,PG-LPS通过增加牙槽骨周围细胞中CathD的表达来影响破骨细胞的形成过程,从而参与牙周炎的进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of cathepsin D induced by Porphyromonas gingivalis lipopolysaccharide in periodontitis

Periodontitis is an inflammatory disease of tooth-supporting tissues caused by oral bacteria. Periodontal ligament loss and alveolar bone destruction occur in progressive periodontitis. Since gingival crevicular fluids (GCF) reflects the inflammatory environment of the periodontal pocket, it is a very important specimen for developing targets for periodontitis diagnosis. An antibody array was performed using GCF collected from healthy participants and patients with periodontitis to identify the proteolytic enzymes involved in periodontitis. Of 21 targets on the antibody array membrane, kallikrein 6 (KLK6), kallikrein 10 (KLK10), cathepsin A (CathA), and cathepsin D (CathD) showed higher levels in periodontitis GCF than in GCF from healthy participants. Lipopolysaccharide stimulation of Porphyromonas gingivalis (PG-LPS) in immortalized gingival fibroblasts only increased CathD protein levels among the four targets. The substrate cleavage activity of CathD was increased in PG-LPS-treated immortalized gingival fibroblast extract. The PG-LPS-induced substrate cleavage effect was abolished by the CathD inhibitor pepstatin A. Osteoclast formation was promoted by treatment with conditioned media from PG-LPS- treated immortalized gingival fibroblasts but inhibited by the CathD inhibitor pepstatin A. These results suggest that PG-LPS affected the osteoclast formation process by increasing CathD expression in cells around the alveolar bone, thereby participating in periodontitis progression.

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来源期刊
European Journal of Oral Sciences
European Journal of Oral Sciences 医学-牙科与口腔外科
CiteScore
3.50
自引率
5.30%
发文量
61
审稿时长
2 months
期刊介绍: The European Journal of Oral Sciences is an international journal which publishes original research papers within clinical dentistry, on all basic science aspects of structure, chemistry, developmental biology, physiology and pathology of relevant tissues, as well as on microbiology, biomaterials and the behavioural sciences as they relate to dentistry. In general, analytical studies are preferred to descriptive ones. Reviews, Short Communications and Letters to the Editor will also be considered for publication. The journal is published bimonthly.
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