外侧缰核和前内侧被盖核对乙醇诱导的位置偏好表达的药理学评价。

IF 1.6 4区 心理学 Q3 BEHAVIORAL SCIENCES
Sukanya G Gakare, Rajesh R Ugale
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引用次数: 0

摘要

尽管乙醇摄入会产生一系列生理效应,但与摄入相关的奖励方面是导致其滥用的主要因素。最近,侧缰(LHb)被证明参与了奖励和厌恶刺激。其主要的谷氨酸能输出,后屈束,投射到前内侧被盖核(RMTg),并控制腹侧被盖区(VTA)多巴胺能系统的活动,以促进奖励回路。虽然已经进行了几次尝试来了解LHb与成瘾之间的关系,但仍然缺乏与乙醇成瘾有关的知识。在本研究中,我们使用条件位置偏好(CPP)测试,通过药物增强或抑制LHb或RMTg神经元的活性,研究了LHb-RMTg逆行束在乙醇诱导的奖励行为中的作用。我们发现LHb内给药l-反式-2,4-吡罗烷二羧酸酯(PDC)(谷氨酸转运蛋白抑制剂)激活LHb谷氨酸能系统显著降低CPP评分;而抑制谷氨酸释放的拉莫三嗪则显著提高CPP评分,对CPP有一定的奖励作用。相反,rmtg内给予muscimol (GABAA受体激动剂)显著增加CPP评分,而bicuculline (GABAA拮抗剂)治疗降低CPP评分。在免疫组化中,我们发现PDC剂量显著降低,而拉莫三嗪治疗显著增加VTA和伏隔核(NAc)的酪氨酸羟化酶免疫反应性(TH-ir)。此外,在rmtg内muscimol给药增加的同时,二胡兰治疗显著降低了VTA和NAc的TH-ir。总之,我们的行为和免疫组织化学结果表明LHb和RMTg在乙醇条件奖励行为表达中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pharmacological evaluation of lateral habenula and rostromedial tegmental nucleus in the expression of ethanol-induced place preference.

Although ethanol administration produces a range of physiological effects, the rewarding aspect associated with its consumption is a major contributory factor to its abuse liability. Recently, lateral habenula (LHb) has been shown to be engaged by both rewarding and aversive stimuli. Its major glutamatergic output, the fasciculus retroflexus, projects to the rostromedial tegmental nucleus (RMTg) and controls the activity of the ventral tegmental area (VTA) dopaminergic system to promote reward circuitry. While several attempts have been made to understand the relationship between LHb and addiction, there is still a lack of knowledge in relation to ethanol addiction. In the present study, by pharmacologically exacerbating or inhibiting the LHb or RMTg neuronal activity during a post-conditioning test, we investigated the role of LHb-RMTg fasciculus retroflexus in ethanol-induced reward behavior using the conditioned place preference (CPP) test. We found that activation of LHb glutamatergic system by intra-LHb administration of l-trans-2,4-pyrrolidine dicarboxylate (PDC) (glutamate transporter inhibitor) significantly decreased CPP score; on the contrary, lamotrigine (inhibits glutamate release) significantly increased CPP score and showed a rewarding effect in CPP. Instead, intra-RMTg administration of muscimol (GABAA receptor agonist) significantly increased CPP score, whereas bicuculline (GABAA antagonist) treatment decreased CPP score. In immunohistochemistry, we found that PDC administration significantly decreased, whereas lamotrigine treatment significantly increased tyrosine hydroxylase immunoreactivity (TH-ir) in VTA and nucleus accumbens (NAc). Furthermore, while intra-RMTg administration of muscimol increased, the bicuculline treatment significantly decreased the TH-ir in VTA and NAc. Together, our behavioral and immunohistochemical results signify the role of LHb and RMTg in the expression of ethanol-conditioned reward behavior.

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来源期刊
Behavioural Pharmacology
Behavioural Pharmacology 医学-行为科学
CiteScore
3.40
自引率
0.00%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Behavioural Pharmacology accepts original full and short research reports in diverse areas ranging from ethopharmacology to the pharmacology of schedule-controlled operant behaviour, provided that their primary focus is behavioural. Suitable topics include drug, chemical and hormonal effects on behaviour, the neurochemical mechanisms under-lying behaviour, and behavioural methods for the study of drug action. Both animal and human studies are welcome; however, studies reporting neurochemical data should have a predominantly behavioural focus, and human studies should not consist exclusively of clinical trials or case reports. Preference is given to studies that demonstrate and develop the potential of behavioural methods, and to papers reporting findings of direct relevance to clinical problems. Papers making a significant theoretical contribution are particularly welcome and, where possible and merited, space is made available for authors to explore fully the theoretical implications of their findings. Reviews of an area of the literature or at an appropriate stage in the development of an author’s own work are welcome. Commentaries in areas of current interest are also considered for publication, as are Reviews and Commentaries in areas outside behavioural pharmacology, but of importance and interest to behavioural pharmacologists. Behavioural Pharmacology publishes frequent Special Issues on current hot topics. The editors welcome correspondence about whether a paper in preparation might be suitable for inclusion in a Special Issue.
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