冷冻电子断层扫描:分辨率革命和原位病毒学发现的激增。

IF 10.4 1区生物学 Q1 BIOPHYSICS

Annual Review of Biophysics Pub Date : 2023-05-09 DOI:10.1146/annurev-biophys-092022-100958

Ye Hong, Yutong Song, Zheyuan Zhang, Sai Li

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引用次数: 4

摘要

近年来，冷冻电子断层扫描(cryo-ET)技术的普及导致了冷冻电子断层扫描分辨率的革命。同时，在提高细胞背景下的靶标识别和低温聚焦离子束(FIB)铣削的吞吐量方面也做出了重大努力。总之，这些进展导致了大量关于包膜病毒如何组装以及病毒如何与受感染宿主细胞相互作用的原位发现。在这篇文章中，我们回顾了冷冻- et技术的最新进展，对病毒组装的高分辨率见解，以及在感染真核和原核细胞内的发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cryo-Electron Tomography: The Resolution Revolution and a Surge of In Situ Virological Discoveries.

The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-ET resolution revolution. Meanwhile, significant efforts have been made to improve the identification of targets in the cellular context and the throughput of cryo-focused ion beam (FIB) milling. Together, these developments led to a surge of in situ discoveries on how enveloped viruses are assembled and how viruses interact with cells in infected hosts. In this article, we review the recent advances in cryo-ET, high-resolution insights into virus assembly, and the findings from inside infected eukaryotic and prokaryotic cells.

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来源期刊

Annual Review of Biophysics 生物-生物物理

CiteScore

21.00

自引率

0.00%

发文量

期刊介绍： The Annual Review of Biophysics, in publication since 1972, covers significant developments in the field of biophysics, including macromolecular structure, function and dynamics, theoretical and computational biophysics, molecular biophysics of the cell, physical systems biology, membrane biophysics, biotechnology, nanotechnology, and emerging techniques.