牛脂肪组织和骨髓干细胞的增殖和成骨分化潜能比较。

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnic & Histochemistry Pub Date : 2023-05-01 DOI:10.1080/10520295.2023.2177347

Ozlem Ozden Akkaya, Tayfun Dikmen, Shah Nawaz, Asm Golam Kibria, Korhan Altunbaş, Artay Yağci, Metin Erdoğan, Mustafa Volkan Yaprakci

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引用次数: 0

摘要

骨髓源性干细胞(Bone marrow stem cells, BMSC)是骨再生领域中应用最多的细胞类型。尽管骨髓间充质干细胞既安全又有效，但对干细胞替代来源的研究仍在继续。利用免疫荧光和PCR技术研究了牛骨髓间充质干细胞和脂肪组织源性间充质干细胞(ATSC)。我们进一步比较了两种干细胞来源的成骨分化潜能。我们在第7、14和21天评估了分化细胞的碱性磷酸酶(ALP)酶水平和钙沉积，以比较两种细胞类型的成骨分化能力。我们发现，在整个成骨分化过程中，ATSC表达的ALP水平明显高于BMSC。第7天和第14天，ATSC组的钙沉积量大于BMSC组。在第21天结束时，BMSC产生了更多的钙沉积。我们发现ATSC比BMSC更快地进行成骨分化，但BMSC在更长的时间内提供更大的矿化。

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Comparison of proliferation and osteogenic differentiation potential of bovine adipose tissue and bone marrow derived stem cells.

Bone marrow derived stem cells (BMSC) are the most utilized cell type in the field of bone regeneration. Although BMSC are both safe and efficacious, the search for alternative sources for stem cells continues. We investigated bovine BMSC and adipose tissue derived mesenchymal stem cells (ATSC) using immunofluorescence and PCR. We further compared the osteogenic differentiation potentials of both sources of stem cells. We assessed alkaline phosphatase (ALP) enzyme levels and calcium deposition in differentiating cells at days 7, 14 and 21 to compare the osteogenic differentiation capability of both cell types. We found that ATSC expressed significantly higher ALP levels compared to BMSC throughout osteogenic differentiation. Calcium deposition was greater in ATSC than BMSC at days 7 and 14. By the end of day 21, BMSC produced greater calcium deposition. We found that ATSC undergo osteogenic differentiation more rapidly than BMSC, but BMSC provide greater mineralization over longer periods.

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.