基于1H核磁共振的代谢组学方法揭示黑姜黄(Curcuma caesia)提取物代谢物变化及其抗氧化和α-葡萄糖苷酶抑制活性的相关性

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nurul Najiha Ain Ibrahim, Nurkhalida Kamal, Ahmed Mediani, Azliana Abu Bakar Sajak, Soo Yee Lee, Khozirah Shaari, Hafeedza Abdul Rahman
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引用次数: 0

摘要

研究背景:姜黄属姜科(Zingiberaceae),是印度和东南亚地区著名的中草药。尽管各种各样的研究结果报道了它们有益的生物活性,但关于姜黄的资料却很少。因此,本研究旨在测定茜草根茎和叶片的酚类物质含量、抗氧化活性和α-葡萄糖苷酶抑制活性。实验方法:采用烘箱干燥法(OD)和冷冻干燥法(FD),分别用Φ(乙醇、水)=100:0、80:20、50:50、0:100的不同提取液进行干燥。采用体外生物活性评价方法,研究了枸杞提取物的生物活性;总酚含量(TPC)、抗氧化剂(DPPH和FRAP)和α-葡萄糖苷酶抑制活性。采用基于质子核磁共振(1H NMR)的代谢组学方法,根据其代谢物谱及其与生物活性的相关性来区分最具活性的提取物。结果与结论:Φ(乙醇、水)=100:0提取的FD根茎具有较强的抑α-葡萄糖苷酶活性(IC50),抑α-葡萄糖苷酶活性分别为(45.4±2.1)mg/g、(147.7±8.3)mg/g和(265.5±38.6)µg/mL,抑α-葡萄糖苷酶活性以没食子酸等量和Trolox等量表达。同时,对DPPH的清除活性,以Φ(乙醇,水)=80:20和100:0提取物的清除活性最高,两者之间无显著差异。因此,选择FD根茎提取物进行进一步的代谢组学分析。主成分分析(PCA)表明不同提取物之间存在明显的区别。偏最小二乘法(PLS)分析结果显示,黄菌根衍生物、1-羟基-1,7-双(4-羟基-3-甲氧基苯基)-(6E)-6-庚烯-3,4-二酮、缬氨酸、木犀草素、zedodiol、β-turmerone、selina-4(15)、7(11)-二烯-8-one、zedoalactone B和germacone的抗氧化活性与α-葡萄糖苷酶抑制活性呈正相关。curdione和1-(4-羟基-3,5-二甲氧基苯基)-7-(4-羟基-3-甲氧基苯基)-(lE,6E)-1,6-庚二烯e3,4-二酮与α-葡萄糖苷酶抑制活性相关。创新与科学贡献:栀子根和叶提取物含有酚类化合物,具有不同的抗氧化能力和α-葡萄糖苷酶抑制能力。这些发现有力地表明,茜草根茎是一种宝贵的天然活性成分来源,可用于制药和食品工业。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<sup>1</sup>H NMR-Based Metabolomics Approach Revealing Metabolite Variation of Black Turmeric (<i>Curcuma caesia</i>) Extracts and Correlation with Its Antioxidant and α-Glucosidase Inhibitory Activities.

<sup>1</sup>H NMR-Based Metabolomics Approach Revealing Metabolite Variation of Black Turmeric (<i>Curcuma caesia</i>) Extracts and Correlation with Its Antioxidant and α-Glucosidase Inhibitory Activities.

<sup>1</sup>H NMR-Based Metabolomics Approach Revealing Metabolite Variation of Black Turmeric (<i>Curcuma caesia</i>) Extracts and Correlation with Its Antioxidant and α-Glucosidase Inhibitory Activities.

1H NMR-Based Metabolomics Approach Revealing Metabolite Variation of Black Turmeric (Curcuma caesia) Extracts and Correlation with Its Antioxidant and α-Glucosidase Inhibitory Activities.

Research background: Curcuma species (Zingiberaceae) are well known medicinal herbs in India and Southeast Asia. Despite various findings reporting their beneficial biological activities, very little information has been recorded on the Curcuma caesia. Thus, this study aims to determine the phenolic content, antioxidant and α-glucosidase inhibitory activity of both rhizome and leaves of C. caesia.

Experimental approach: Rhizome and leaves of C. caesia were dried with oven (OD) and freeze (FD)-drying methods, and extracted with different Φ(ethanol,water)=100:0, 80:20, 50:50 and 0:100. The bioactivities of C. caesia extracts were evaluated using in vitro tests; total phenolic content (TPC), antioxidant (DPPH and FRAP) and α-glucosidase inhibitory activity. Proton nuclear magnetic resonance (1H NMR)-based metabolomics approach was employed to differentiate the most active extracts based on their metabolite profiles and correlation with bioactivities.

Results and conclusions: The FD rhizome extracted with Φ(ethanol,water)=100:0 was observed to have potent TPC expressed as gallic acid equivalents, FRAP expressed as Trolox equivalents and α-glucosidase inhibitory activity with values of (45.4±2.1) mg/g extract, (147.7±8.3) mg/g extract and (265.5±38.6) µg/mL (IC50), respectively. Meanwhile, for DPPH scavenging activity, the Φ(ethanol,water)=80:20 and 100:0 extracts of FD rhizome showed the highest activity with no significant difference between them. Hence, the FD rhizome extracts were selected for further metabolomics analysis. Principal component analysis (PCA) showed clear discrimination among the different extracts. Partial least square (PLS) analysis showed positive correlations of the metabolites, including xanthorrhizol derivative, 1-hydroxy-1,7-bis(4-hydroxy-3-methoxyphenyl)-(6E)-6-heptene-3,4-dione, valine, luteolin, zedoardiol, β-turmerone, selina-4(15),7(11)-dien-8-one, zedoalactone B and germacrone, with the antioxidant and α-glucosidase inhibition activities, whereas curdione and 1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-(lE,6E)-1,6-heptadiene3,4-dione were correlated with α-glucosidase inhibitory activity.

Novelty and scientific contribution: C. caesia rhizome and leaf extracts contained phenolic compounds and had varies antioxidant and α-glucosidase inhibitory capacities. These findings strongly suggest that the rhizomes of C. caesia are an invaluable natural source of active ingredients for applications in pharmaceutical and food industries.

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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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