微小RNA靶向ErbB信号通路基因在心肌细胞分化中的意义

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Akram Gholipour , Ali Zahedmehr , Farshad Shakerian , Shiva Irani , Maziar Oveisee , Seyed Javad Mowla , Mahshid Malakootian
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引用次数: 0

摘要

目的心肌细胞分化是一个复杂的过程,伴随着基因表达的改变。ErbB信号通路对于心脏发育的各个阶段是必需的。我们的目的是通过计算机方法鉴定靶向ErbB信号通路基因的潜在微小RNA。方法从GSE108021中获得心肌细胞分化的小RNA测序数据。通过DESeq2包装获得差异表达的miRNA。确定了所鉴定的miRNA的信号通路和基因本体过程,并确定了影响ErbB信号通路的那些miRNA的靶向基因。结果高差异表达的miRNA在分化阶段之间很常见,它们靶向参与ErbB信号通路的基因如下:let-7g-5p同时靶向CDKN1A和NRAS,而let-7c-5p和let-7d-5p仅靶向CDKN1A和NRAS。let-7家族成员靶向MAPK8和ABL2。GSK3B被miR-199a-5p和miR-214-3p靶向,ERBB4被miR-199b-3p和miR-653-5p靶向。miR-214-3p、miR-199b-3p、miR-1277-5p、miR-21-5p和miR-21-3p分别靶向CBL、mTOR、Jun、JNKK和GRB1。MAPK8被miR-214-3p靶向,ABL2也被miR-125b-5p和miR-1277-5p靶向。结论我们确定了心肌细胞发育和心脏病理生理学进展中ErbB信号通路中的miRNA及其靶基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Significance of microRNA-targeted ErbB signaling pathway genes in cardiomyocyte differentiation

Objective(s)

Cardiomyocyte differentiation is a complex process that follows the progression of gene expression alterations. The ErbB signaling pathway is necessary for various stages of cardiac development. We aimed to identify potential microRNAs targeting the ErbB signaling pathway genes by in silico approaches.

Methods

Small RNA-sequencing data were obtained from GSE108021 for cardiomyocyte differentiation. Differentially expressed miRNAs were acquired via the DESeq2 package. Signaling pathways and gene ontology processes for the identified miRNAs were determined and the targeted genes of those miRNAs affecting the ErbB signaling pathway were determined.

Results

Results revealed highly differentially expressed miRNAs were common between the differentiation stages and they targeted the genes involved in the ErbB signaling pathway as follows: let-7g-5p targets both CDKN1A and NRAS, while let-7c-5p and let-7d-5p hit CDKN1A and NRAS exclusively. let-7 family members targeted MAPK8 and ABL2. GSK3B was targeted by miR-199a-5p and miR-214-3p, and ERBB4 was targeted by miR-199b-3p and miR-653-5p. miR-214-3p, miR-199b-3p, miR-1277-5p, miR-21-5p, and miR-21-3p targeted CBL, mTOR, Jun, JNKK, and GRB1, respectively. MAPK8 was targeted by miR-214-3p, and ABL2 was targeted by miR-125b-5p and miR-1277-5p, too.

Conclusion

We determined miRNAs and their target genes in the ErbB signaling pathway in cardiomyocyte development and consequently heart pathophysiology progression.

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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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