{"title":"慢性旋毛虫病对实验大鼠弓形虫 RH 毒株的免疫调节作用。","authors":"Abeer E Saad, Dalia S Ashour, Eman Rashad","doi":"10.1080/20477724.2023.2191233","DOIUrl":null,"url":null,"abstract":"<p><p>Mixed parasitic infections could affect the host immunological responses and re-design the pathogenesis of each other. The impact of <i>Toxoplasma gondii</i> (<i>T. gondii</i>) and <i>Trichinella spiralis</i> (<i>T. spiralis</i>) co-infection on the immune response remains unclear. The objective of the present study was to investigate the possible effect of chronic trichinellosis on the immune response of rats infected with <i>T. gondii</i> virulent RH strain. Animals were divided into four groups: group I: non-infected negative control; group II: infected with <i>T. spiralis</i>; group III: infected with <i>T. gondii</i> and group IV: infected with <i>T. spiralis</i> then infected with <i>T. gondii</i> 35 days post <i>T. spiralis</i> infection (co-infected group). The interaction between <i>T. spiralis</i> and <i>T. gondii</i> was evaluated by histopathological examination of liver and brain tissues, immunohistochemical expression of inducible nitric oxide synthase (iNOS), and β-catenin in the brain tissues, and CD4<sup>+</sup> and CD8<sup>+</sup> T cells percentages, and tumor necrosis factor (TNF)-alpha expression in the spleen tissues. Along with, splenic interleukin (IL)-4 and IL-10 mRNA expression levels were measured 15 days post-<i>Toxoplasma</i> infection. Our study revealed that prior infection with <i>T. spiralis</i> leads to attenuation of Th1 response against <i>T. gondii</i>, including iNOS, TNF-α, and CD8<sup>+</sup> T-cell response with improvement of the histopathological changes in the tissues. In conclusion, in the co-infected rats, a balanced immune response has been developed with the end result, improvement of the histopathological changes in the liver and brain.</p>","PeriodicalId":19850,"journal":{"name":"Pathogens and Global Health","volume":"117 4","pages":"417-434"},"PeriodicalIF":4.9000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177679/pdf/","citationCount":"0","resultStr":"{\"title\":\"Immunomodulatory effects of chronic trichinellosis on <i>Toxoplasma gondii</i> RH virulent strain in experimental rats.\",\"authors\":\"Abeer E Saad, Dalia S Ashour, Eman Rashad\",\"doi\":\"10.1080/20477724.2023.2191233\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mixed parasitic infections could affect the host immunological responses and re-design the pathogenesis of each other. The impact of <i>Toxoplasma gondii</i> (<i>T. gondii</i>) and <i>Trichinella spiralis</i> (<i>T. spiralis</i>) co-infection on the immune response remains unclear. The objective of the present study was to investigate the possible effect of chronic trichinellosis on the immune response of rats infected with <i>T. gondii</i> virulent RH strain. Animals were divided into four groups: group I: non-infected negative control; group II: infected with <i>T. spiralis</i>; group III: infected with <i>T. gondii</i> and group IV: infected with <i>T. spiralis</i> then infected with <i>T. gondii</i> 35 days post <i>T. spiralis</i> infection (co-infected group). The interaction between <i>T. spiralis</i> and <i>T. gondii</i> was evaluated by histopathological examination of liver and brain tissues, immunohistochemical expression of inducible nitric oxide synthase (iNOS), and β-catenin in the brain tissues, and CD4<sup>+</sup> and CD8<sup>+</sup> T cells percentages, and tumor necrosis factor (TNF)-alpha expression in the spleen tissues. Along with, splenic interleukin (IL)-4 and IL-10 mRNA expression levels were measured 15 days post-<i>Toxoplasma</i> infection. Our study revealed that prior infection with <i>T. spiralis</i> leads to attenuation of Th1 response against <i>T. gondii</i>, including iNOS, TNF-α, and CD8<sup>+</sup> T-cell response with improvement of the histopathological changes in the tissues. In conclusion, in the co-infected rats, a balanced immune response has been developed with the end result, improvement of the histopathological changes in the liver and brain.</p>\",\"PeriodicalId\":19850,\"journal\":{\"name\":\"Pathogens and Global Health\",\"volume\":\"117 4\",\"pages\":\"417-434\"},\"PeriodicalIF\":4.9000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10177679/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathogens and Global Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/20477724.2023.2191233\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/3/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens and Global Health","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/20477724.2023.2191233","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/3/15 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Immunomodulatory effects of chronic trichinellosis on Toxoplasma gondii RH virulent strain in experimental rats.
Mixed parasitic infections could affect the host immunological responses and re-design the pathogenesis of each other. The impact of Toxoplasma gondii (T. gondii) and Trichinella spiralis (T. spiralis) co-infection on the immune response remains unclear. The objective of the present study was to investigate the possible effect of chronic trichinellosis on the immune response of rats infected with T. gondii virulent RH strain. Animals were divided into four groups: group I: non-infected negative control; group II: infected with T. spiralis; group III: infected with T. gondii and group IV: infected with T. spiralis then infected with T. gondii 35 days post T. spiralis infection (co-infected group). The interaction between T. spiralis and T. gondii was evaluated by histopathological examination of liver and brain tissues, immunohistochemical expression of inducible nitric oxide synthase (iNOS), and β-catenin in the brain tissues, and CD4+ and CD8+ T cells percentages, and tumor necrosis factor (TNF)-alpha expression in the spleen tissues. Along with, splenic interleukin (IL)-4 and IL-10 mRNA expression levels were measured 15 days post-Toxoplasma infection. Our study revealed that prior infection with T. spiralis leads to attenuation of Th1 response against T. gondii, including iNOS, TNF-α, and CD8+ T-cell response with improvement of the histopathological changes in the tissues. In conclusion, in the co-infected rats, a balanced immune response has been developed with the end result, improvement of the histopathological changes in the liver and brain.
期刊介绍：
Pathogens and Global Health is a journal of infectious disease and public health that focuses on the translation of molecular, immunological, genomics and epidemiological knowledge into control measures for global health threat. The journal publishes original innovative research papers, reviews articles and interviews policy makers and opinion leaders on health subjects of international relevance. It provides a forum for scientific, ethical and political discussion of new innovative solutions for controlling and eradicating infectious diseases, with particular emphasis on those diseases affecting the poorest regions of the world.
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