预防性经皮穴位电刺激对阿尔茨海默病模型大鼠抗氧化性认知障碍的改善作用

IF 2.8 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Heng Weng , Qing Wang , Ran Ye, Yamei Bai, Hui Yang, Guihua Xu, Qiuqin Wang
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引用次数: 0

摘要

背景阿尔茨海默病是一种慢性、不可逆的神经退行性疾病。氧化应激出现在AD早期。经皮穴位电刺激(TEAS)是一种将中医穴位与电刺激相结合的无创治疗方法,不良反应少。本研究旨在探讨预防性TEAS治疗(P-TEAS)对AD模型大鼠认知障碍和氧化应激的改善作用。方法SD大鼠颈后皮下注射D-半乳糖(D-gal,120mg/kg/D)9周,建立AD模型,模拟AD早期的氧化应激。第10周第1天,双侧海马CA1区注射Aβ1-42(1μg/μl)。P-TEAS从皮下注射D-gal的第一天开始同步,持续9周。结果实验结果表明,P-TEAS能提高AD模型大鼠在Morris水迷宫中的空间记忆能力。超氧化物歧化酶(SOD)在P-TEAS组中上调。通过检测抗氧化应激信号通路,即Kelch样ECH相关蛋白1(Keap1)/NFE2相关因子2(Nrf2),发现P-TEAS可促进Nrf2进入细胞核,并上调保护因子血红素加氧酶1(HO-1)和NADPH醌氧化还原酶1(NQO1)的产生。还发现P-TEAS可以下调BCL2相关X蛋白(Bax)、胱天蛋白酶3和胱天蛋白酶9的表达,以抑制神经元凋亡。结论sP-TEAS在预防AD发生发展方面具有与电针相似的疗效。P-TEAS是一种新的预防AD的非侵入性干预疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Anti-oxidative-initiated cognitive impairment amelioration in Alzheimer's disease model rats through preventive transcutaneous electrical acupoint stimulation

Anti-oxidative-initiated cognitive impairment amelioration in Alzheimer's disease model rats through preventive transcutaneous electrical acupoint stimulation

Background

Alzheimer's disease (AD) is a chronic and irreversible neurodegenerative disease. Oxidative stress emerges at the early AD stage. As a non-invasive therapy with few adverse reactions, transcutaneous electrical acupoint stimulation (TEAS) combines acupuncture points of traditional Chinese medicine (TCM) and electrical stimulation. This study aimed to investigate the amelioration effects of preventive TEAS treatment (P-TEAS) on cognitive impairment and oxidative stress in AD model rats.

Methods

The AD model was established via subcutaneous injections of D-galactose (D-gal, 120 mg/kg/d) into the back of neck for 9 weeks in Sprague Dawley (SD) rats to simulate the oxidative stress in the early AD stage. On the first day of the 10th week, Aβ1–42 (1 μg/μl) was injected into the CA1 regions of the bilateral hippocampus. P-TEAS was synchronized from the first day of subcutaneous D-gal injections for 9 weeks.

Results

Empirical measurements showed that P-TEAS can improve the spatial memory ability of AD model rats in the Morris water maze. Superoxide dismutase (SOD) was upregulated in the P-TEAS group. Through the detection of the anti-oxidative stress signaling pathway, namely, Kelch-like ECH-associated protein 1 (Keap1)/ NFE2-related factor 2 (Nrf2), it was found that P-TEAS could promote Nrf2 entering into the nucleus and upregulating the production of protective factors heme oxygenase 1 (HO-1) and NADPH quinone oxidoreductase 1 (NQO1). It was also found that P-TEAS could downregulate the expressions of BCL2-associated X-protein (Bax), caspase 3, and caspase 9 to inhibit neuronal apoptosis.

Conclusions

P-TEAS has similar efficacy to electroacupuncture in preventing AD occurrence and development. P-TEAS is a new non-invasive intervention therapy for the prevention of AD.

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来源期刊
Integrative Medicine Research
Integrative Medicine Research Medicine-Complementary and Alternative Medicine
CiteScore
6.50
自引率
2.90%
发文量
65
审稿时长
12 weeks
期刊介绍: Integrative Medicine Research (IMR) is a quarterly, peer-reviewed journal focused on scientific research for integrative medicine including traditional medicine (emphasis on acupuncture and herbal medicine), complementary and alternative medicine, and systems medicine. The journal includes papers on basic research, clinical research, methodology, theory, computational analysis and modelling, topical reviews, medical history, education and policy based on physiology, pathology, diagnosis and the systems approach in the field of integrative medicine.
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