Govania unica gen. nov., sp. nov.,一种罕见的生物圈细菌,代表了α变形菌纲中的一个新家族

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Peter Vandamme , Charlotte Peeters , Amanda Hettiarachchi , Margo Cnockaert , Aurélien Carlier
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引用次数: 0

摘要

菌株LMG 31809 T从比利时温带混交林表层土壤样品中分离得到。将其16S rRNA基因序列与已发表的有效名称的细菌类型菌株进行比较,将其定位为Alphaproteobacteria纲,并突出了其与邻近物种(Emcibacterales和Sphingomonadales)的主要进化差异。相同土壤样品的16S rRNA扩增子测序显示,该群落高度多样化,其中酸杆菌门和Alphaproteobacteria占主导地位。但未能产生与菌株LMG 31809 T高度相似的扩增子序列变异。没有对应于同一物种的宏基因组组装基因组,对公开的16S rRNA扩增子测序数据集的综合分析表明,菌株LMG 31809 T是一种罕见的生物圈细菌,在多个土壤和水相关生态系统中丰度很低。基因组分析表明,该菌株是一种严格的有氧异养菌,它是一种无糖水解菌,以有机酸和可能的芳香族化合物为生长基质。我们建议将LMG 31809 T分类为Alphaproteobacteria Govaniaceae新科Govania unica gen. nov., sp. nov中的一个新种。其型菌株为LMG 31809 T (=CECT 30155 T),菌株LMG 31809 T全基因组序列大小为3.21 Mbp。G + C含量为58.99 mol%。菌株LMG 31809 T的16S rRNA基因和全基因组序列已公开获取,登录号分别为OQ161091和JANWOI000000000。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Govania unica gen. nov., sp. nov., a rare biosphere bacterium that represents a novel family in the class Alphaproteobacteria

Strain LMG 31809 T was isolated from a top soil sample of a temperate, mixed deciduous forest in Belgium. Comparison of its 16S rRNA gene sequence with that of type strains of bacteria with validly published names positioned it in the class Alphaproteobacteria and highlighted a major evolutionary divergence from its near neighbor species which represented species of the orders Emcibacterales and Sphingomonadales. 16S rRNA amplicon sequencing of the same soil sample revealed a highly diverse community in which Acidobacteria and Alphaproteobacteria predominated, but failed to yield amplicon sequence variants highly similar to that of strain LMG 31809 T. There were no metagenome assembled genomes that corresponded to the same species and a comprehensive analysis of public 16S rRNA amplicon sequencing data sets demonstrated that strain LMG 31809 T represents a rare biosphere bacterium that occurs at very low abundances in multiple soil and water-related ecosystems. The genome analysis suggested that this strain is a strictly aerobic heterotroph that is asaccharolytic and uses organic acids and possibly aromatic compounds as growth substrates. We propose to classify LMG 31809 T as a novel species within a novel genus, Govania unica gen. nov., sp. nov, within the novel family Govaniaceae of the class Alphaproteobacteria. Its type strain is LMG 31809 T (=CECT 30155 T). The whole-genome sequence of strain LMG 31809 T has a size of 3.21 Mbp. The G + C content is 58.99 mol%. The 16S rRNA gene and whole-genome sequences of strain LMG 31809 T are publicly available under accession numbers OQ161091 and JANWOI000000000, respectively.

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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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