饲料中10种毛霉烯同时定量的实验室间研究。

IF 2.6 4区医学 Q2 MYCOLOGY

Mycotoxin Research Pub Date : 2023-05-01 DOI:10.1007/s12550-023-00476-4

Masayo Nomura, Kenji Shidara, Iyo Yasuda

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引用次数: 0

摘要

在8个实验室开展实验室间研究，探讨饲料中HT-2毒素(HT-2)、T-2毒素(T-2)、二乙酰氧基雪腐镰刀醇(DAS)、新茄醇(NES)、3-乙酰脱氧雪腐镰刀醇(3-AcDON)、15-乙酰脱氧雪腐镰刀醇(15-AcDON)、脱氧雪腐镰刀醇(DON)、脱氧雪腐镰刀醇-3-葡萄糖苷(D3G)、雪腐镰刀醇(NIV)和fusarenon-X (FUS-X)的同时定量方法。样品中的真菌毒素用有水乙腈提取，用多功能柱(InertSep®VRA-3)和磷脂去除柱(Hybrid SPE®-磷脂)纯化，然后用常压化学电离模式的液相色谱-串联质谱(LC-MS/MS)进行定量。A型毛线虫(HT-2、T-2、DAS和NES)的平均回收率、重复性、再现性和Horwitz比值分别为99.8 ~ 109%、3.1 ~ 9.8%、4.3 ~ 9.8%和0.19 ~ 0.45。B型霉菌毒素(3-AcDON、15-AcDON、DON、NIV和FUS-X)的检测值分别为89.9 ~ 116%、3.4 ~ 9.1%、5.6 ~ 14%和0.25 ~ 0.70，改性霉菌毒素(D3G)的检测值分别为78.2 ~ 96.7%、3.5 ~ 6.4%和13 ~ 22%。

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Inter-laboratory study on simultaneous quantification of ten trichothecenes in feed.

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Inter-laboratory study on simultaneous quantification of ten trichothecenes in feed.

An inter-laboratory study was performed in eight laboratories to evaluate the simultaneous quantification method for HT-2 toxin (HT-2), T-2 toxin (T-2), diacetoxyscirpenol (DAS), neosolaniol (NES), 3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), deoxynivalenol (DON), deoxynivalenol-3-glucoside (D3G), nivalenol (NIV), and fusarenon-X (FUS-X) in feed. The mycotoxins in the samples were extracted with hydrous acetonitrile, purified using a multifunctional column (InertSep^® VRA-3) and a phospholipid removal column (Hybrid SPE^®-Phospholipid), and then quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with atmospheric pressure chemical ionisation mode. The mean recovery, repeatability, reproducibility, and Horwitz ratio from the inter-laboratory validation study were 99.8-109%, 3.1-9.8%, 4.3-9.8%, and 0.19-0.45, respectively, for type A trichothecenes (HT-2, T-2, DAS, and NES). Those values for type B trichothecenes (3-AcDON, 15-AcDON, DON, NIV, and FUS-X) were 89.9-116%, 3.4-9.1%, 5.6-14%, and 0.25-0.70, and the values for modified mycotoxin (D3G) were 78.2-96.7%, 3.5-6.4%, and 13-22%, respectively.

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来源期刊

Mycotoxin Research MYCOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

6.40

自引率

6.70%

发文量

期刊介绍： Mycotoxin Research, the official publication of the Society for Mycotoxin Research, is a peer-reviewed, scientific journal dealing with all aspects related to toxic fungal metabolites. The journal publishes original research articles and reviews in all areas dealing with mycotoxins. As an interdisciplinary platform, Mycotoxin Research welcomes submission of scientific contributions in the following research fields: - Ecology and genetics of mycotoxin formation - Mode of action of mycotoxins, metabolism and toxicology - Agricultural production and mycotoxins - Human and animal health aspects, including exposure studies and risk assessment - Food and feed safety, including occurrence, prevention, regulatory aspects, and control of mycotoxins - Environmental safety and technology-related aspects of mycotoxins - Chemistry, synthesis and analysis.