绵羊外周血单个核细胞对细粒棘球蚴microRNA-71过度表达的反应分析

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI:10.1016/j.molbiopara.2023.111556

Yating Li , Lujun Yan , Duojie Ci , Rui Li , Wanjing Li , Tianqi Xia , Hengzhi Shi , Mazhar Ayaz , Yadong Zheng , Pu Wang

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引用次数: 0

摘要

由细粒棘球蚴引起的囊性棘球蚴病仍然是一种人畜共患疾病，对公众健康和肉类生产行业构成巨大威胁。感染颗粒大肠杆菌的绵羊血清中egr-miR-71的丰度相对较高，但其作用尚不清楚。利用生物信息学、细胞迁移和Transwell分析，我们比较分析了绵羊PBMC对egr-miR-71过表达的蛋白质组和细胞侵袭。结果表明，egr-miR-71共诱导157种蛋白质差异表达，主要参与免疫反应。在绵羊PBMC中，与对照组相比，egr-miRNA-71过表达诱导巨噬细胞迁移抑制因子（MIF）显著下调，从而促进细胞迁移和侵袭。该结果将为进一步研究循环egr-miR-71在颗粒大肠杆菌感染期间免疫反应中的作用提供线索。

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Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression

Cyst echinococcosis, caused by Echinococcus granulosus, remains a zoonotic disease posing a great threat to public health and meat production industry. Sheep infected with E. granulosus show relatively high abundance of egr-miR-71 in the sera, but its role is unknown. Using bioinformatics and cell migration and Transwell assays, we comparatively analyzed the proteomes and cell invasion of sheep PBMCs in response to egr-miR-71 overexpression. The results showed that the egr-miR-71 induced a total of 157 proteins being differentially expressed and mainly involved in immune responses. In sheep PBMCs, egr-miRNA-71 overexpression induced significant downregulation of macrophage migration inhibitory factor (MIF) and accordingly promoted cell migration and invasion compared with the control. The results will provide a clue for further investigation of a role of circulating egr-miR-71 in immune responses during E. granulosus infection.

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.