白藜芦醇;保存血小板细胞功能的双刃剑抗氧化剂分子的见解。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Abbas Khosravi, Mohammad Reza Deyhim, Fatemeh Yari, Mahin Nikougoftar Zarif
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引用次数: 0

摘要

背景:本研究旨在探讨白藜芦醇对血小板浓缩物(PCs)的剂量依赖性影响。我们也试图找到这种作用的分子机制。方法:收集伊朗输血组织(IBTO)提供的pc。共研究了10个pc。将PCs分为4组,分别为未治疗组(对照组)和不同剂量白藜芦醇治疗组;10、30、50µM。血小板聚集和总活性氧(ROS)水平在PCs储存第3天进行评估。进行了计算机分析,以找出可能涉及的机理。结果:各组血小板对胶原蛋白的聚集量均显著下降,但对照组血小板聚集量明显高于治疗组(p结论:白藜芦醇对血小板聚集的影响呈剂量依赖性。此外,我们还发现白藜芦醇在控制细胞氧化状态方面起着双刃剑的作用。因此,使用最佳剂量的白藜芦醇是非常重要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Resveratrol; a Double-Edged Sword Antioxidant Agent for Preserving Platelet Cell Functions During Storage; Molecular Insights.

Background: In the current study we have aimed to find the effects of Resveratrol treatment on platelet concentrates (PCs) at the dose dependent manner. We have also attempted to find the molecular mechanism of the effects.

Methods: The PCs, have received from Iranian blood transfusion organization (IBTO). Totally 10 PCs were studied. The PCs divided into 4 groups including untreated (control) and treated by different dose of Resveratrol; 10, 30 and 50 µM. Platelet aggregation and total reactive oxygen species (ROS) levels were evaluated at day 3 of PCs storage. In silico analysis was carried out to find out the potential involved mechanisms.

Results: The aggregation against collagen has fallen dramatically in all studied groups but at the same time, aggregation was significantly higher in the control versus treated groups (p<0.05). The inhibitory effect was dose dependent. The aggregation against Ristocetin did not significantly affect by Resveratrol treatment. The mean of total ROS significantly increased in all studied groups except those PCs treated with 10 µM of Resveratrol (P=0.9). The ROS level significantly increased with increasing Resveratrol concentration even more than control group (slope=11.6, P=0.0034). Resveratrol could potently interact with more than 15 different genes which, 10 of them enrolled in cellular regulation of the oxidative stress.

Conclusions: Our findings indicated that the Resveratrol affect the platelet aggregation at the dose dependent manner. Moreover, we have also found that the Resveratrol play as double-edged sword in the controlling oxidative state of the cells. Therefore, Using the optimal dose of Resveratrol is the great of importance.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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