甲基化分析作为一种辅助工具在细胞学诊断罕见的肛门生殖器病变-一个试点研究。

Q4 Medicine
Ceskoslovenska patologie Pub Date : 2023-01-01
Jana Němcová, Kateřina Černá, Radek Šíma, Iva Kinkorová Luňáčková, Filip Rob, Petr Martínek, Jiří Bouda, Jana, Šmahelová, Ondrej Ondič
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引用次数: 0

摘要

某些细胞基因的甲基化沉默是癌变进展的标志,因此检测甲基化的测试可用于恶性疾病的诊断或分期。宫颈癌的鳞状细胞癌几乎100%是由高风险人乳头瘤病毒(HR-HPV)的长期感染引起的,在宫颈癌的诊断中,某些细胞基因的甲基化沉默是晚期发育不良病变的高度特异性标志物,似乎是由病毒癌蛋白E6和E7异常激活甲基转移酶DNMT1引起的。在宫颈阴道细胞学标本上进行甲基化试验可以增加这种非侵入性试验的诊断价值,并选择严重鳞状细胞病变的患者进行随访。由HR-HPV引起的其他不太常见的肛门生殖器恶性肿瘤在较小程度上也可以通过细胞学检查检测到-各种来源的腺体病变,最常见的是宫颈和子宫内膜腺癌和肛门癌。本初步研究的目的是评估甲基化测试在诊断这些恶性肿瘤中的效用,该队列包括50例腺病变宫颈阴道细胞学检查和74例肛门细胞学检查,这些细胞学检查来自与肛门癌发展高风险男性发生性行为的hiv阳性男性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Methylation analysis as an auxiliary tool in cytological diagnostics of infrequent anogenital lesions - a pilot study.

Methylation silencing of certain cellular genes is a sign of carcinogenesis progression and therefore tests that detect methylation could be used in the diagnosis or staging of malignant diseases. In the diagnosis of squamous cell carcinomas of the cervix which are almost 100% caused by long-term infection with highrisk human papillomavirus (HR-HPV), methylation silencing of certain cellular genes is a highly specific marker of advanced dysplastic lesions and appears to result from aberrant activation of the methyltransferase DNMT1 by viral oncoproteins E6 and E7. A methylation test performed on a cervicovaginal cytology specimen allows to increase the diagnostic value of this non-invasive test and to select patients with severe squamous cell lesions for follow-up. Other less frequent anogenital malignancies that are induced by HR-HPV to a lesser extent can also be detected by cytological examination - glandular lesions of various origins, most commonly cervical and endometrial adenocarcinomas and anal carcinoma. The aim of our pilot study was to evaluate the utility of a methylation test for the diagnosis of these malignancies in a cohort of 50 liquid-based cervicovaginal cytologies with glandular lesion and 74 liquid-based anal cytologies from HIV-positive men having sex with men who are at high risk for anal cancer development.

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来源期刊
Ceskoslovenska patologie
Ceskoslovenska patologie Medicine-Medicine (all)
CiteScore
0.40
自引率
0.00%
发文量
17
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