高弹性三氧化二矿聚集体及其对 M1 和 M2 巨噬细胞活力和粘附性、吞噬细胞活性、活性氧的产生以及细胞因子的影响。

Restorative Dentistry & Endodontics Pub Date : 2022-12-29 eCollection Date: 2023-02-01 DOI:10.5395/rde.2023.48.e6
Betânia Canal Vasconcellos, Layara Cristine Tomaz Tavares, Danilo Couto da Silva, Francielen Oliveira Fonseca, Francine Benetti, Antônio Paulino Ribeiro Sobrinho, Warley Luciano Fonseca Tavares
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引用次数: 0

摘要

目的:本研究评估了高弹性三氧化二铝矿物骨料(MTA-HP)与白色 MTA(Angelus)相比对 M1 和 M2 巨噬细胞活性的影响:与白色 MTA(Angelus)相比,本研究评估了高弹性三氧化二铝矿物骨料(MTA-HP)对 M1 和 M2 巨噬细胞活性的影响:在测试材料存在的情况下培养腹膜炎症 M1(来自 C57BL/6 小鼠)和 M2(来自 BALB/c 小鼠)巨噬细胞。对细胞存活率(MTT 和胰蓝试验)、粘附力、吞噬能力、活性氧(ROS)产生量、肿瘤坏死因子(TNF)-α 和转化生长因子(TGF)-β 产生量进行了评估。采用参数方差分析和非参数 Kruskal-Wallis 检验。当 p < 0.05 时,结果被认为是有意义的:MTT 试验显示,MTA-HP 在 24 小时内以及 MTA 和 MTA-HP 之后的 M1 代谢显著下降。胰蓝试验显示,与 MTA 相比,MTA-HP 在 48 小时时活 M1 明显减少,在 48 和 72 小时时活 M2 明显减少。与对照组相比,两种材料的 M1 和 M2 附着力和吞噬作用无明显差异。Zymosan A 可刺激巨噬细胞产生 ROS。在没有干扰素-γ的情况下,M1产生的TNF-α在组间没有显著差异。就 M2 而言,两种材料在有刺激的情况下都能产生较高的 TNF-α,但组间差异不明显。同样,M1和M2巨噬细胞产生的TGF-β在组间也无明显差异:结论:在不同的时间点,M1 和 M2 巨噬细胞对 MTA 和 MTA-HP 的存活率不同。在 MTA 载体中加入增塑剂不会干扰 M1 和 M2 巨噬细胞的活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.

High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.

High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.

High-plasticity mineral trioxide aggregate and its effects on M1 and M2 macrophage viability and adherence, phagocyte activity, production of reactive oxygen species, and cytokines.

Objectives: This study evaluated the effects of high-plasticity mineral trioxide aggregate (MTA-HP) on the activity of M1 and M2 macrophages, compared to white MTA (Angelus).

Materials and methods: Peritoneal inflammatory M1 (from C57BL/6 mice) and M2 (from BALB/c mice) macrophages were cultured in the presence of the tested materials. Cell viability (MTT and trypan blue assays), adhesion, phagocytosis, reactive oxygen species (ROS) production, and tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β production were evaluated. Parametric analysis of variance and the non-parametric Kruskal-Wallis test were used. Results were considered significant when p < 0.05.

Results: The MTT assay revealed a significant decrease in M1 metabolism with MTA-HP at 24 hours, and with MTA and MTA-HP later. The trypan blue assay showed significantly fewer live M1 at 48 hours and live M2 at 48 and 72 hours with MTA-HP, compared to MTA. M1 and M2 adherence and phagocytosis showed no significant differences compared to control for both materials. Zymosan A stimulated ROS production by macrophages. In the absence of interferon-γ, TNF-α production by M1 did not significantly differ between groups. For M2, both materials showed higher TNF-α production in the presence of the stimulus, but without significant between-group differences. Likewise, TGF-β production by M1 and M2 macrophages was not significantly different between the groups.

Conclusions: M1 and M2 macrophages presented different viability in response to MTA and MTA-HP at different time points. Introducing a plasticizer into the MTA vehicle did not interfere with the activity of M1 and M2 macrophages.

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