异氟醚滴注法麻醉小鼠。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-05-05 DOI:10.1177/00236772231169550
Maya J Bodnar, Anna S Ratuski, Daniel M Weary
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引用次数: 1

摘要

建议在二氧化碳安乐死之前用异氟醚麻醉,但可以限制蒸发器的使用。汽化器的另一种替代方法是“滴法”,将固定体积的异氟烷引入感应室。先前的研究表明,通过滴注法以5%浓度给药的异氟醚是有效的,但对小鼠有反感;更低的浓度还没有测试过。我们用滴法对浓度低于5%的异氟醚诱导小鼠的行为和不敏感进行了评估。雄性Crl:CD-1 (ICR)小鼠(n = 27)随机分配到三种异氟烷浓度:1.7%、2.7%和3.7%中的一种。在诱导过程中,记录麻木和压力相关行为的测量。所有小鼠都达到了手术麻醉平面,暴露于高浓度的小鼠更快达到手术麻醉平面;当浓度从1.7增加到2.7和3.7%时,平躺时间(最小二乘法平均值±SE分别为120.5 s±8.1、97.9 s±8.1和82.8 s±8.1)、翻正反射丧失(分别为149.1 s±8.5、127.7 s±8.5和1000.7 s±8.5)和退足反射丧失(分别为214.5 s±8.3、172.2 s±8.3和146.4 s±8.3)均有所下降。养育是最常见的与压力相关的行为,并且在所有治疗中使用异氟醚后立即最明显。结果表明,异氟醚浓度低至1.7%时,滴注法可有效麻醉小鼠;未来的工作应该评估老鼠厌恶。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mouse isoflurane anesthesia using the drop method.

Anesthesia with isoflurane prior to carbon dioxide euthanasia is recommended as a refinement, but vaporizer access can be limited. An alternative to vaporizers is the 'drop' method, introducing a fixed volume of isoflurane into the induction chamber. Previous work suggests that isoflurane administered at a concentration of 5% via the drop method is effective but aversive to mice; lower concentrations have not been tested. We assessed mouse behavior and insensibility with induction using the drop method for isoflurane concentrations below 5%. Male Crl:CD-1 (ICR) mice (n = 27) were randomly allocated to one of three isoflurane concentrations: 1.7%, 2.7%, and 3.7%. During induction, measures of insensibility and stress-related behaviors were recorded. All mice reached a surgical plane of anesthesia, and mice exposed to higher concentrations did so more quickly; as concentrations increased from 1.7 to 2.7 and 3.7%, the time to recumbency (Least squares means ± SE: 120.5 s ± 8.1, 97.9 s ± 8.1, and 82.8 s ± 8.1, respectively), loss of righting reflex (149.1 s ± 8.5, 127.7 s ± 8.5, and 100.7 s ± 8.5, respectively), and loss of pedal withdrawal reflex (214.5 s ± 8.3, 172.2 s ± 8.3, and 146.4 s ± 8.3, respectively) all declined. Rearing was the most frequently performed stress-related behavior, and was most pronounced immediately following isoflurane administration for all treatments. Our results indicate that the drop method can be used to effectively anesthetize mice with isoflurane concentrations as low as 1.7%; future work should assess mouse aversion.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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