Inas M Abou El-Enain, Nermine N Abed, Eman E Helal, Eman S Abdelkhalek, Waleed Suleiman, Nesreen A Safwat, Mohammed Yosri
{"title":"具有抗白色念珠菌和抗肿瘤活性的灰色链霉菌生态合成Ag-NPs。","authors":"Inas M Abou El-Enain, Nermine N Abed, Eman E Helal, Eman S Abdelkhalek, Waleed Suleiman, Nesreen A Safwat, Mohammed Yosri","doi":"10.2174/2772434418666230427165013","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The most significant sexually transmissible fungal disease, semen candidiasis, is caused by Candida albicans and impacts male reproductive potential. Actinomycetes are a group of microorganisms that could be isolated from various habitats and used for the biosynthesis of various nanoparticles with biomedical applications.</p><p><strong>Objective: </strong>Testing antifungal activity of biosynthesized Ag nanoparticles versus isolated C. albicans from semen as well as its anticancer activity versus the Caco-2 cell line.</p><p><strong>Methods: </strong>Screening 17 isolated actinomycetes for the biosynthesis of Ag nanoparticle biosynthesis. Characterization of biosynthesized nanoparticles, testing its anti-Candida albicans, and antitumor activity.</p><p><strong>Results: </strong>Streptomyces griseus was the isolate that identified silver nanoparticles using UV, FTIR, XRD and TEM. Biosynthesized nanoparticles have promising anti-Candida albicans with MIC (125 ± 0.8) µg/ml and accelerate apoptotic rate versus Caco-2 cells (IC50 = 7.30 ± 0.54 µg/ml) with minimal toxicity (CC50 = 142.74 ± 4.71 µg/ml) versus Vero cells.</p><p><strong>Conclusion: </strong>Certain actinomycetes could be used for the biosynthesis of nanoparticles with successive antifungal and anticancer activity to be verified by in vivo studies.</p>","PeriodicalId":74643,"journal":{"name":"Recent advances in anti-infective drug discovery","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Eco-friendly Biosynthesis of Ag-NPs by Streptomyces griseus With Anti- Candida albicans and Antitumor Activity.\",\"authors\":\"Inas M Abou El-Enain, Nermine N Abed, Eman E Helal, Eman S Abdelkhalek, Waleed Suleiman, Nesreen A Safwat, Mohammed Yosri\",\"doi\":\"10.2174/2772434418666230427165013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The most significant sexually transmissible fungal disease, semen candidiasis, is caused by Candida albicans and impacts male reproductive potential. Actinomycetes are a group of microorganisms that could be isolated from various habitats and used for the biosynthesis of various nanoparticles with biomedical applications.</p><p><strong>Objective: </strong>Testing antifungal activity of biosynthesized Ag nanoparticles versus isolated C. albicans from semen as well as its anticancer activity versus the Caco-2 cell line.</p><p><strong>Methods: </strong>Screening 17 isolated actinomycetes for the biosynthesis of Ag nanoparticle biosynthesis. Characterization of biosynthesized nanoparticles, testing its anti-Candida albicans, and antitumor activity.</p><p><strong>Results: </strong>Streptomyces griseus was the isolate that identified silver nanoparticles using UV, FTIR, XRD and TEM. Biosynthesized nanoparticles have promising anti-Candida albicans with MIC (125 ± 0.8) µg/ml and accelerate apoptotic rate versus Caco-2 cells (IC50 = 7.30 ± 0.54 µg/ml) with minimal toxicity (CC50 = 142.74 ± 4.71 µg/ml) versus Vero cells.</p><p><strong>Conclusion: </strong>Certain actinomycetes could be used for the biosynthesis of nanoparticles with successive antifungal and anticancer activity to be verified by in vivo studies.</p>\",\"PeriodicalId\":74643,\"journal\":{\"name\":\"Recent advances in anti-infective drug discovery\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Recent advances in anti-infective drug discovery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/2772434418666230427165013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Recent advances in anti-infective drug discovery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/2772434418666230427165013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Eco-friendly Biosynthesis of Ag-NPs by Streptomyces griseus With Anti- Candida albicans and Antitumor Activity.
Background: The most significant sexually transmissible fungal disease, semen candidiasis, is caused by Candida albicans and impacts male reproductive potential. Actinomycetes are a group of microorganisms that could be isolated from various habitats and used for the biosynthesis of various nanoparticles with biomedical applications.
Objective: Testing antifungal activity of biosynthesized Ag nanoparticles versus isolated C. albicans from semen as well as its anticancer activity versus the Caco-2 cell line.
Methods: Screening 17 isolated actinomycetes for the biosynthesis of Ag nanoparticle biosynthesis. Characterization of biosynthesized nanoparticles, testing its anti-Candida albicans, and antitumor activity.
Results: Streptomyces griseus was the isolate that identified silver nanoparticles using UV, FTIR, XRD and TEM. Biosynthesized nanoparticles have promising anti-Candida albicans with MIC (125 ± 0.8) µg/ml and accelerate apoptotic rate versus Caco-2 cells (IC50 = 7.30 ± 0.54 µg/ml) with minimal toxicity (CC50 = 142.74 ± 4.71 µg/ml) versus Vero cells.
Conclusion: Certain actinomycetes could be used for the biosynthesis of nanoparticles with successive antifungal and anticancer activity to be verified by in vivo studies.
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