食用菌 Mycoleptodonoides aitchisonii(Bunaharitake)单核和双核中 A 交配型基因的鉴定、表征和表达。

IF 1.5 4区 生物学 Q4 MYCOLOGY
Mycoscience Pub Date : 2021-03-20 eCollection Date: 2021-01-01 DOI:10.47371/mycosci.2020.11.008
Rini Riffiani, Fu-Chia Chen, Weitong Zhang, Takayuki Wada, Norihiro Shimomura, Takeshi Yamaguchi, Tadanori Aimi
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引用次数: 0

摘要

确定Mycoleptodonoides aitchisonii的交配类型对于促进这种食用菌的育种和栽培非常重要。为了弄清金针菇双极交配系统的分子机制,研究人员对同源结构域蛋白基因2(Mahd2)进行了鉴定。M. aitchisonii 50005-18 株系中的 Mahd2 基因组 DNA 片段长 1,851 bp,编码 614 个氨基酸的蛋白质。转录分析表明,在产生钳夹细胞的单核菌株中,Mahd2的表达量高于不产生钳夹细胞的单核菌株。在能够形成真正钳形细胞的单核菌株 TUFC 50005-4 中,Mahd2 的相对表达水平最高。这些结果表明,钳形细胞的形成受 A 交配型同源染色体蛋白的调控,Mahd2 基因的高表达可能会促进钳形细胞的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification, characterization and expression of <i>A</i>-mating type genes in monokaryons and dikaryons of the edible mushroom <i>Mycoleptodonoides aitchisonii</i> (Bunaharitake).

Identification, characterization and expression of <i>A</i>-mating type genes in monokaryons and dikaryons of the edible mushroom <i>Mycoleptodonoides aitchisonii</i> (Bunaharitake).

Identification, characterization and expression of <i>A</i>-mating type genes in monokaryons and dikaryons of the edible mushroom <i>Mycoleptodonoides aitchisonii</i> (Bunaharitake).

Identification, characterization and expression of A-mating type genes in monokaryons and dikaryons of the edible mushroom Mycoleptodonoides aitchisonii (Bunaharitake).

Identifying the mating-type in Mycoleptodonoides aitchisonii is important for enhancing breeding and cultivation of this edible mushroom. To clarify the molecular mechanisms of the bipolar mating system in M. aitchisonii, the homeodomain protein gene 2 (Mahd2) was characterized. A genomic DNA fragment of Mahd2 in M. aitchisonii 50005-18 strain was 1,851 bp long and encoded a protein of 614 amino acids. Transcriptional analysis revealed that the expression of Mahd2 was higher in monokaryotic strains that produced clamp cells than in those that did not. The highest relative expression level of Mahd2 was observed in monokaryon TUFC 50005-4, which was capable of forming a true clamp. These results suggested that the formation of clamp cells is regulated by A-mating type homeodomain proteins, and the frequency of clamp cell formation might be promoted by high expression of the Mahd2 gene.

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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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