{"title":"柽柳菇麦角硫因含量遗传连锁图谱的构建及数量性状位点的检测。","authors":"Shozo Yoneyama, Kaede Maeda, Ayuka Sadamori, Sayaka Saitoh, Mayumi Tsuda, Tomonori Azuma, Atsushi Nagano, Takahiro Tomiyama, Teruyuki Matsumoto","doi":"10.47371/mycosci.2020.11.003","DOIUrl":null,"url":null,"abstract":"<p><p>Developing high-content strains of L-ergothioneine (EGT), an antioxidant amino acid, is an important breeding target for tamogitake mushroom, <i>Pleurotus cornucopiae</i> var. <i>citrinopileatus</i>. We constructed a genetic linkage map based on segregation analysis of markers in 105 F1 progenies. The loci of 245 markers, including 10 AFLP markers, 195 Rad markers, 2 mating type factors, and 38 gene markers, were mapped. The map contained 12 linkage groups with a total genetic distance of 906.8 cM, and an average marker interval of 4.0 cM. The population from crossing between tester monokaryon and F1 progenies was used to characterize quantitative trait loci (QTL) for EGT content. With composite interval mapping (CIM) method, QTL of EGT content were found to be located in linkage group 10, having a Logarithm of the odds (LOD) score of 2.53 with a 10.1% contribution rate. Moreover, a single nucleotide polymorphism (SNP), A/T, was identified in a gene region of the genome in the neighborhood where the QTL peak existed. This SNP genotype was in good agreement with the EGT phenotypes of each strain in the both QTL population and wild population. Thus, this SNP would have great potential value to use the marker-assisted selection (MAS) for this mushroom with high EGT content.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/58/MYC-62-071.PMC9157747.pdf","citationCount":"0","resultStr":"{\"title\":\"Construction of a genetic linkage map and detection of quantitative trait locus for the ergothioneine content in tamogitake mushroom (<i>Pleurotus cornucopiae</i> var. <i>citrinopileatus</i>).\",\"authors\":\"Shozo Yoneyama, Kaede Maeda, Ayuka Sadamori, Sayaka Saitoh, Mayumi Tsuda, Tomonori Azuma, Atsushi Nagano, Takahiro Tomiyama, Teruyuki Matsumoto\",\"doi\":\"10.47371/mycosci.2020.11.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Developing high-content strains of L-ergothioneine (EGT), an antioxidant amino acid, is an important breeding target for tamogitake mushroom, <i>Pleurotus cornucopiae</i> var. <i>citrinopileatus</i>. We constructed a genetic linkage map based on segregation analysis of markers in 105 F1 progenies. The loci of 245 markers, including 10 AFLP markers, 195 Rad markers, 2 mating type factors, and 38 gene markers, were mapped. The map contained 12 linkage groups with a total genetic distance of 906.8 cM, and an average marker interval of 4.0 cM. The population from crossing between tester monokaryon and F1 progenies was used to characterize quantitative trait loci (QTL) for EGT content. With composite interval mapping (CIM) method, QTL of EGT content were found to be located in linkage group 10, having a Logarithm of the odds (LOD) score of 2.53 with a 10.1% contribution rate. Moreover, a single nucleotide polymorphism (SNP), A/T, was identified in a gene region of the genome in the neighborhood where the QTL peak existed. This SNP genotype was in good agreement with the EGT phenotypes of each strain in the both QTL population and wild population. Thus, this SNP would have great potential value to use the marker-assisted selection (MAS) for this mushroom with high EGT content.</p>\",\"PeriodicalId\":18780,\"journal\":{\"name\":\"Mycoscience\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/58/MYC-62-071.PMC9157747.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycoscience\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.47371/mycosci.2020.11.003\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycoscience","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.47371/mycosci.2020.11.003","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
开发高含量的抗氧化氨基酸l -麦角硫因(EGT)菌株是柽柳菇(Pleurotus cornucopiae变种. citrinopileatus)的重要育种目标。通过对105个F1后代的标记分离分析,构建了遗传连锁图谱。共定位了245个标记位点,其中AFLP标记10个,Rad标记195个,交配型因子2个,基因标记38个。该图谱包含12个连锁群,总遗传距离为906.8 cM,平均标记间隔为4.0 cM。利用测试单核子与F1后代杂交群体进行EGT含量定量性状位点(QTL)的鉴定。利用复合区间映射(CIM)方法,发现EGT含量的QTL位于连锁组10,LOD分数的对数为2.53,贡献率为10.1%。此外,在QTL峰附近的基因组基因区域发现了单核苷酸多态性(SNP) a /T。该SNP基因型与各菌株在QTL群体和野生群体中的EGT表型一致。因此,这一SNP对于利用标记辅助选择(MAS)对这种EGT含量高的蘑菇具有很大的潜在价值。
Construction of a genetic linkage map and detection of quantitative trait locus for the ergothioneine content in tamogitake mushroom (Pleurotus cornucopiae var. citrinopileatus).
Developing high-content strains of L-ergothioneine (EGT), an antioxidant amino acid, is an important breeding target for tamogitake mushroom, Pleurotus cornucopiae var. citrinopileatus. We constructed a genetic linkage map based on segregation analysis of markers in 105 F1 progenies. The loci of 245 markers, including 10 AFLP markers, 195 Rad markers, 2 mating type factors, and 38 gene markers, were mapped. The map contained 12 linkage groups with a total genetic distance of 906.8 cM, and an average marker interval of 4.0 cM. The population from crossing between tester monokaryon and F1 progenies was used to characterize quantitative trait loci (QTL) for EGT content. With composite interval mapping (CIM) method, QTL of EGT content were found to be located in linkage group 10, having a Logarithm of the odds (LOD) score of 2.53 with a 10.1% contribution rate. Moreover, a single nucleotide polymorphism (SNP), A/T, was identified in a gene region of the genome in the neighborhood where the QTL peak existed. This SNP genotype was in good agreement with the EGT phenotypes of each strain in the both QTL population and wild population. Thus, this SNP would have great potential value to use the marker-assisted selection (MAS) for this mushroom with high EGT content.
期刊介绍:
Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.