抗小鼠CXCR6单克隆抗体Cx6Mab-1的2 ×丙氨酸取代法表位定位

Q3 Medicine

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-02-01 DOI:10.1089/mab.2022.0029

Yu Isoda, Tomohiro Tanaka, Hiroyuki Suzuki, Teizo Asano, Takeo Yoshikawa, Kaishi Kitamura, Yuma Kudo, Ryo Ejima, Kazuki Ozawa, Mika K Kaneko, Yukinari Kato

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引用次数: 1

摘要

一种抗小鼠CXC趋化因子受体6 (mCXCR6)单克隆抗体Cx6Mab-1已被研制成功。Cx6Mab-1适用于流式细胞术、Western blotting和酶联免疫吸附测定。本研究的目的是利用2 ×丙氨酸突变的mCXCR6确定Cx6Mab-1的结合表位。流式细胞术分析显示Cx6Mab-1不能识别mCXCR6的S8A-A9G、L10A-Y11A、D12A-G13A和H14A-Y15A突变体。结果清楚地表明Cx6Mab-1的结合表位包括mCXCR6的Ser8、Ala9、Leu10、Tyr11、Asp12、Gly13、His14和Tyr15。Cx6Mab-1表位的成功确定可能有助于mCXCR6的病理生理研究。

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Epitope Mapping Using the Cell-Based 2 × Alanine Substitution Method About the Anti-mouse CXCR6 Monoclonal Antibody, Cx₆Mab-1.

An anti-mouse CXC chemokine receptor 6 (mCXCR6) monoclonal antibody (mAb), Cx6Mab-1, was developed recently. Cx6Mab-1 is applicable for flow cytometry, Western blotting, and enzyme-linked immunosorbent assay. The purpose of this study is to determine the binding epitope of Cx6Mab-1 using 2 × alanine mutated mCXCR6. Analysis of flow cytometry revealed that Cx6Mab-1 did not recognize S8A-A9G, L10A-Y11A, D12A-G13A, and H14A-Y15A mutants of mCXCR6. The results clearly indicate that the binding epitope of Cx6Mab-1 includes Ser8, Ala9, Leu10, Tyr11, Asp12, Gly13, His14, and Tyr15 of mCXCR6. The successful determination of the Cx6Mab-1 epitope might contribute to the pathophysiological investigation of mCXCR6.

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来源期刊

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Medicine-Immunology and Allergy

CiteScore

4.80

自引率

0.00%

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