全新中心染色体上satdna的进化:来自半翅目昆虫Mahanarva属的见解。

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Allison Anjos, Diogo Milani, Vanessa B Bardella, Andressa Paladini, Diogo C Cabral-de-Mello
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引用次数: 2

摘要

卫星dna (satdna)是真核生物基因组的主要组成部分之一,参与染色体的组织和多样化。尽管对其进行了大量研究,但关于它们在全新中心物种(即弥散着丝粒)上的进化信息却很少,由于重复序列组织的差异,弥散着丝粒可能导致不同的进化模式。本文采用生物信息学和细胞遗传学相结合的方法,对Mahanarva全新中心昆虫的卫星体进化进行了研究。在两个物种中,重新鉴定发现了大量的satdna,分别为110和113个,单体长度范围为18-4228 bp。结果表明,四刺田鼠的satdna总丰度为6.67%,特异田鼠的satdna总丰度为1.98%,但共有satdna的丰度不同。在其他Mahanarva上对M. quadripunctata和M. spectabilis最丰富的重复序列进行染色体定位,加强了satdna的动态性质。注意到satdna的染色体分布模式不同,在不同数量的染色体和不同位置上出现簇,以及出现分散信号或非簇的satdna。总之,我们的数据证明了Mahanarva中satdna的高度动态,这一基因组片段参与了该属的染色体多样化。satdna的总体特征和进化模式与单中心染色体相似,这表明与单中心染色体相比,在全新中心染色体上观察到的基因组室的差异组织对satdna的进化没有太大的影响。以比较的方式分析其他全新中心物种的卫星组将有助于阐明这一问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evolution of satDNAs on holocentric chromosomes: insights from hemipteran insects of the genus Mahanarva.

Evolution of satDNAs on holocentric chromosomes: insights from hemipteran insects of the genus Mahanarva.

Satellite DNAs (satDNAs) constitute one of the main components of eukaryote genomes and are involved in chromosomal organization and diversification. Although largely studied, little information was gathered about their evolution on holocentric species, i.e., diffuse centromeres, which, due to differences in repeat organization, could result in different evolutionary patterns. Here, we combined bioinformatics and cytogenetic approaches to evaluate the evolution of the satellitomes in Mahanarva holocentric insects. In two species, de novo identification revealed a high number of satDNAs, 110 and 113, with an extreme monomer length range of 18-4228 bp. The overall abundance of satDNAs was observed to be 6.67% in M. quadripunctata and 1.98% in M. spectabilis, with different abundances for the shared satDNAs. Chromosomal mapping of the most abundant repeats of M. quadripunctata and M. spectabilis on other Mahanarva reinforced the dynamic nature of satDNAs. Variable patterns of chromosomal distribution for the satDNAs were noticed, with the occurrence of clusters on distinct numbers of chromosomes and at different positions and the occurrence of scattered signals or nonclustered satDNAs. Altogether, our data demonstrated the high dynamism of satDNAs in Mahanarva with the involvement of this genomic fraction in chromosome diversification of the genus. The general characteristics and patterns of evolution of satDNAs are similar to those observed on monocentric chromosomes, suggesting that the differential organization of genome compartments observed on holocentric chromosomes compared with monocentric chromosomes does not have a large impact on the evolution of satDNAs. Analysis of the satellitomes of other holocentric species in a comparative manner will shed light on this issue.

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来源期刊
Chromosome Research
Chromosome Research 生物-生化与分子生物学
CiteScore
4.70
自引率
3.80%
发文量
31
审稿时长
1 months
期刊介绍: Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to: · Chromosomes and their linkage to diseases; · Chromosome organization within the nucleus; · Chromatin biology (transcription, non-coding RNA, etc); · Chromosome structure, function and mechanics; · Chromosome and DNA repair; · Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting, dosage compensation, sex determination, chromosome remodeling); · Architectural/epigenomic organization of the genome; · Functional annotation of the genome; · Functional and comparative genomics in plants and animals; · Karyology studies that help resolve difficult taxonomic problems or that provide clues to fundamental mechanisms of genome and karyotype evolution in plants and animals; · Mitosis and Meiosis; · Cancer cytogenomics.
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