阐明口腔微生物组及其与宿主的相互作用:分子微生物学研究的工具和方法。

IF 10.1 2区 生物学 Q1 MICROBIOLOGY
Justin Merritt, Jens Kreth
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引用次数: 0

摘要

过去十年中,DNA 测序技术的进步激发了人们对人类微生物组的空前兴趣,这主要是由于发现人类疾病的广泛多样性与微生物组失调有关。这些研究的直接结果是,大量未被充分研究和表征的微生物被确定为粘膜健康和疾病的潜在驱动因素。该领域迫在眉睫的挑战是如何将这些观察结果转化为共生和菌群失调的分子机理研究。为了应对这一挑战,许多这些新发现的微生物将需要调整以用于实验模型。因此,本综述全面概述了在人类口腔微生物群细菌遗传研究中发挥关键作用的分子微生物学工具和技术。在此,我们将使用口腔微生物组文献中的具体实例来说明支持这些技术的生物学原理、为什么该领域需要这些技术以及这些技术是如何实施的。希望这些信息能成为有用的参考指南,帮助促进对人体不同粘膜部位发现的许多未充分研究和未定性的新物种进行分子微生物学研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.

Advancements in DNA sequencing technologies within the last decade have stimulated an unprecedented interest in the human microbiome, largely due the broad diversity of human diseases found to correlate with microbiome dysbiosis. As a direct consequence of these studies, a vast number of understudied and uncharacterized microbes have been identified as potential drivers of mucosal health and disease. The looming challenge in the field is to transition these observations into defined molecular mechanistic studies of symbiosis and dysbiosis. In order to meet this challenge, many of these newly identified microbes will need to be adapted for use in experimental models. Consequently, this review presents a comprehensive overview of the molecular microbiology tools and techniques that have played crucial roles in genetic studies of the bacteria found within the human oral microbiota. Here, we will use specific examples from the oral microbiome literature to illustrate the biology supporting these techniques, why they are needed in the field, and how such technologies have been implemented. It is hoped that this information can serve as a useful reference guide to help catalyze molecular microbiology studies of the many new understudied and uncharacterized species identified at different mucosal sites in the body.

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来源期刊
FEMS microbiology reviews
FEMS microbiology reviews 生物-微生物学
CiteScore
17.50
自引率
0.90%
发文量
45
审稿时长
6-12 weeks
期刊介绍: Title: FEMS Microbiology Reviews Journal Focus: Publishes reviews covering all aspects of microbiology not recently surveyed Reviews topics of current interest Provides comprehensive, critical, and authoritative coverage Offers new perspectives and critical, detailed discussions of significant trends May contain speculative and selective elements Aimed at both specialists and general readers Reviews should be framed within the context of general microbiology and biology Submission Criteria: Manuscripts should not be unevaluated compilations of literature Lectures delivered at symposia must review the related field to be acceptable
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