顺铂诱导大鼠成体肾干/祖细胞衍生肾类器官细胞死亡

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Yonago acta medica Pub Date : 2023-02-01 DOI:10.33160/yam.2023.02.018

Yasushi Kuromi, Shinji Kitamura, Takeshi Endo, Shota Ueno, Kenji Kokura, Tetsuya Ohbayashi

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引用次数: 0

摘要

背景:利用干细胞/祖细胞衍生的类器官来评估化学物质的毒性已经广泛增加。具有肾元样结构的类器官(NLS)可以通过三维培养从大鼠成体肾干/祖细胞(rKS细胞)中获得。在这项研究中，我们研究了顺铂(一种体内诱导肾毒性的抗癌药物)对rKS细胞源性NLS的影响。方法:在Matrigel基质中培养rKS细胞，同时获得12个类器官。采用显微成像技术测量各类器官的表面积，采用图像分析方法监测NLS的形态学变化。NLS暴露于顺铂，并评估其相关效应。结果:NLS随时间延长。12个类器官的表面积几乎是恒定的。顺铂暴露诱导NLS细胞死亡，抑制类器官表面积的增加。结论:顺铂暴露可引起rKS细胞NLS损伤。因此，类器官可能是一个有价值的体外模型，以评估肾毒性。

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Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids.

Background: The use of stem/ progenitor cell-derived organoids to evaluate the toxicity of chemical substances has widely increased. Organoids with nephron-like structures (NLS) can be derived from rat adult kidney stem/ progenitor cells (rKS cells) using three-dimensional culture. In this study, we examined the effects of cisplatin, an anticancer drug that induces nephrotoxicity in vivo, on rKS cell-derived NLS.

Methods: Twelve organoids were simultaneously derived from three-dimensionally cultured rKS cells in Matrigel matrices. The surface area of each organoid was measured using microscopy-based imaging, and the morphological changes of NLS were monitored using an image analysis method. NLS were exposed to cisplatin, and their associated effects were assessed.

Results: NLS elongated over time. The surface areas of the 12 organoids were almost constant. Cisplatin exposure induced cell death in NLS and inhibited the increase in the surface area of the organoids.

Conclusion: Cisplatin exposure induces damage to NLS derived from rKS cells. Thus, the organoids may be valuable as an in vitro model to assess nephrotoxicity.

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来源期刊

Yonago acta medica MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Yonago Acta Medica (YAM) is an electronic journal specializing in medical sciences, published by Tottori University Medical Press, 86 Nishi-cho, Yonago 683-8503, Japan. The subject areas cover the following: molecular/cell biology; biochemistry; basic medicine; clinical medicine; veterinary medicine; clinical nutrition and food sciences; medical engineering; nursing sciences; laboratory medicine; clinical psychology; medical education. Basically, contributors are limited to members of Tottori University and Tottori University Hospital. Researchers outside the above-mentioned university community may also submit papers on the recommendation of a professor, an associate professor, or a junior associate professor at this university community. Articles are classified into four categories: review articles, original articles, patient reports, and short communications.