作为平衡计划的一部分,在两个辐照设施进行中子照射后生物剂量测定的国际比较工作。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Cytogenetic and Genome Research Pub Date : 2023-01-01 Epub Date: 2023-04-18 DOI:10.1159/000530728
David Endesfelder, Ulrike Kulka, Martin Bucher, Ulrich Giesen, Guy Garty, Christina Beinke, Matthias Port, Gaetan Gruel, Eric Gregoire, Georgia Terzoudi, Sotiria Triantopoulou, Elizabeth A Ainsbury, Jayne Moquet, Mingzhu Sun, María Jesús Prieto, Mercedes Moreno Domene, Joan-Francesc Barquinero, Monica Pujol-Canadell, Anne Vral, Ans Baeyens, Andrzej Wojcik, Ursula Oestreicher
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引用次数: 0

摘要

在放射性或核事件的情况下,生物剂量学可以是一个重要的工具,以支持临床决策。在核事件中,个体可能暴露在中子和光子的混合场中。电场的组成和中子能谱对染色体的损伤程度有影响。在跨大西洋BALANCE项目期间,模拟了距离震中1.5公里处类似广岛装置的照射,并进行了基于双心染色体的生物剂量测定,以评估参与者发现未知剂量的能力,并测试中子谱差异的影响。在第一步中,通过在德国(PTB)和美国(CINF)的两个不同设施中以0 Gy至4 Gy范围内的5种剂量照射血液样品建立校准曲线。这些样本被送到来自RENEB网络的8个参与实验室,每个参与者对双中心染色体进行评分。接下来,血液样本分别在两个设施中进行4次盲剂量照射,并发送给参与者,根据已建立的校准曲线提供剂量估计。评估了人工和半自动双中心染色体评分对中子暴露的适用性。此外,还比较了两种辐照装置产生的中子的生物有效性。CINF辐照样品的校准曲线显示,与PTB辐照样品相比,CINF辐照样品的生物有效性高1.4倍。对于双中心染色体的人工评分,根据项目中建立的校准曲线,大多数测试样品的剂量被成功地分辨出来。对于半自动评分,测试样品的剂量估计不太成功。校正曲线显示剂量与双心计数弥散指数呈非线性关系,特别是人工评分。不同辐照设施生物效应的差异表明,中子能谱对双心计数有很大影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
International Comparison Exercise for Biological Dosimetry after Exposures with Neutrons Performed at Two Irradiation Facilities as Part of the BALANCE Project.

In the case of a radiological or nuclear event, biological dosimetry can be an important tool to support clinical decision-making. During a nuclear event, individuals might be exposed to a mixed field of neutrons and photons. The composition of the field and the neutron energy spectrum influence the degree of damage to the chromosomes. During the transatlantic BALANCE project, an exposure similar to a Hiroshima-like device at a distance of 1.5 km from the epicenter was simulated, and biological dosimetry based on dicentric chromosomes was performed to evaluate the participants ability to discover unknown doses and to test the influence of differences in neutron spectra. In a first step, calibration curves were established by irradiating blood samples with 5 doses in the range of 0-4 Gy at two different facilities in Germany (Physikalisch-Technische Bundesanstalt [PTB]) and the USA (the Columbia IND Neutron Facility [CINF]). The samples were sent to eight participating laboratories from the RENEB network and dicentric chromosomes were scored by each participant. Next, blood samples were irradiated with 4 blind doses in each of the two facilities and sent to the participants to provide dose estimates based on the established calibration curves. Manual and semiautomatic scoring of dicentric chromosomes were evaluated for their applicability to neutron exposures. Moreover, the biological effectiveness of the neutrons from the two irradiation facilities was compared. The calibration curves from samples irradiated at CINF showed a 1.4 times higher biological effectiveness compared to samples irradiated at PTB. For manual scoring of dicentric chromosomes, the doses of the test samples were mostly successfully resolved based on the calibration curves established during the project. For semiautomatic scoring, the dose estimation for the test samples was less successful. Doses >2 Gy in the calibration curves revealed nonlinear associations between dose and dispersion index of the dicentric counts, especially for manual scoring. The differences in the biological effectiveness between the irradiation facilities suggested that the neutron energy spectrum can have a strong impact on the dicentric counts.

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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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