听力研究中豚鼠全身性耳聋的改进:卡那霉素和速尿经腿静脉联合给药引起的感音神经性听力损失。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES
Laboratory Animals Pub Date : 2023-12-01 Epub Date: 2023-04-18 DOI:10.1177/00236772231167679
Wiebke Behrends, Daniel Ahrens, Jens P Bankstahl, Karl-Heinz Esser, Gerrit Paasche, Thomas Lenarz, Verena Scheper
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引用次数: 0

摘要

听力障碍对全世界的人口有很大的影响。近年来,对理解和治疗听力障碍的研究显著增加。在此背景下,最相关的动物物种之一是豚鼠,它必须失聪才能研究几种听力病理并开发新的治疗方法。在听力研究中,卡那霉素皮下注射和速尿静脉注射是一种长期存在的方法,可导致永久性听力丧失,而无需在耳部进行手术干预。静脉应用速尿需要在动物颈部进行侵入性手术,以暴露颈静脉,因为必须在大约2.5分钟的时间内注射相对较大的体积(每500g体重1ml)。我们已经建立了一个更温和的替代方案通过穿刺腿部静脉使用速尿。为此,定制的套管针装置被建造,以允许静脉穿刺和随后缓慢注射速尿。该入路在11只豚鼠身上进行了试验,分别经前腿经头前臂静脉和后腿经隐静脉入路。在手术前后分别测量频率特异性听力阈值,以验证听力正常和耳聋成功。这种新的系统性耳聋方法在11只动物中的10只动物中成功实施。隐静脉是最适合的应用。由于动物的情况,腿后静脉应用,比暴露颈静脉致聋的动物更好,因此假设的减少动物压力的改进被认为是成功的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Refinement of systemic guinea pig deafening in hearing research: Sensorineural hearing loss induced by co-administration of kanamycin and furosemide via the leg veins.

Auditory disabilities have a large impact on the human population worldwide. Research into understanding and treating hearing disabilities has increased significantly in recent years. One of the most relevant animal species in this context is the guinea pig, which has to be deafened to study several of the hearing pathologies and develop novel therapies. Applying kanamycin subcutaneously and furosemide intravenously is a long-established method in hearing research, leading to permanent hearing loss without surgical intervention at the ear. The intravenous application of furosemide requires invasive surgery in the cervical area of the animals to expose the jugular vein, since a relatively large volume (1 ml per 500 g body weight) must be injected over a period of about 2.5 min. We have established a gentler alternative by applying the furosemide by puncture of the leg veins. For this, custom-made cannula-needle devices were built to allow the vein puncture and subsequent slow injection of the furosemide. This approach was tested in 11 guinea pigs through the foreleg via the cephalic antebrachial vein and through the hind leg via the saphenous vein. Frequency-specific hearing thresholds were measured before and after the procedure to verify normal hearing and successful deafening, respectively. The novel approach of systemic deafening was successfully implemented in 10 out of 11 animals. The Vena saphena was best suited to the application. Since the animals' condition, post leg vein application, was better in comparison to animals deafened by exposure of the Vena jugularis, the postulated refinement that reduced animal stress was deemed successful.

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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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