Ivana Vrca, Dina Ramić, Željana Fredotović, Sonja Smole Možina, Ivica Blažević, Tea Bilušić
{"title":"Tropaeolum majus L. var. altum种子精油和提取物的化学成分和生物活性。","authors":"Ivana Vrca, Dina Ramić, Željana Fredotović, Sonja Smole Možina, Ivica Blažević, Tea Bilušić","doi":"10.17113/ftb.60.04.22.7667","DOIUrl":null,"url":null,"abstract":"<p><strong>Research background: </strong>Plant <i>Tropaeolum majus</i> L. (garden nasturtium) belongs to the family Tropaeolaceae and contains benzyl glucosinolate. The breakdown product of benzyl glucosinolate, benzyl isothiocyanate (BITC), exhibits various biological activities such as antiproliferative, antibacterial and antiinflammatory. In order to optimize the content of biologically active volatile compounds in plant extract and essential oil, the use of appropriate extraction technique has a crucial role.</p><p><strong>Experimental approach: </strong>The current study investigates the effect of two modern extraction methods, microwave-assisted distillation (MAD) and microwave hydrodiffusion and gravity (MHG), on the chemical composition of volatile components present in the essential oil and extract of garden nasturtium (<i>T. majus</i> L. <i>var. altum</i>) seeds. Investigation of the biological activity of samples (essential oil, extract and pure compounds) was focused on the antiproliferative effect against different cancer cell lines: cervical cancer cell line (HeLa), human colon cancer cell line (HCT116) and human osteosarcoma cell line (U2OS), and the antibacterial activity which was evaluated against the growth and adhesion of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> to polystyrene surface.</p><p><strong>Results and conclusions: </strong>Essential oil and extract of garden nasturtium (<i>T. majus</i>) seeds were isolated by two extraction techniques: MAD and MHG. BITC and benzyl cyanide (BCN) present in the extract were identified by gas chromatography-mass spectrometry. Essential oil of <i>T. majus</i> showed higher antiproliferative activity (IC<sub>50</sub><5 µg/mL) than <i>T. majus</i> extract (IC<sub>50</sub><27 µg/mL) against three cancer cell lines: HeLa, HCT116 and U2OS. BITC showed much higher inhibitory effect on all tested cells than BCN. The essential oil and extract of <i>T. majus</i> showed strong antimicrobial activity against <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Novelty and scientific contribution: </strong>This work represents the first comparative report on the antiproliferative activity of the essential oil and extract of <i>T. majus</i> seeds, BITC and BCN against HeLa, HCT116 and U2OS cells as well as their antimicrobial activity against <i>S. aureus</i> and <i>E. coli</i>. This study demonstrates that the essential oil of <i>T. majus</i> seeds exhibits stronger antiproliferative and antimicrobial activity than the plant extract.</p>","PeriodicalId":12400,"journal":{"name":"Food Technology and Biotechnology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901342/pdf/","citationCount":"0","resultStr":"{\"title\":\"Chemical Composition and Biological Activity of Essential Oil and Extract from the Seeds of <i>Tropaeolum majus</i> L. var. <i>altum</i>.\",\"authors\":\"Ivana Vrca, Dina Ramić, Željana Fredotović, Sonja Smole Možina, Ivica Blažević, Tea Bilušić\",\"doi\":\"10.17113/ftb.60.04.22.7667\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Research background: </strong>Plant <i>Tropaeolum majus</i> L. (garden nasturtium) belongs to the family Tropaeolaceae and contains benzyl glucosinolate. The breakdown product of benzyl glucosinolate, benzyl isothiocyanate (BITC), exhibits various biological activities such as antiproliferative, antibacterial and antiinflammatory. In order to optimize the content of biologically active volatile compounds in plant extract and essential oil, the use of appropriate extraction technique has a crucial role.</p><p><strong>Experimental approach: </strong>The current study investigates the effect of two modern extraction methods, microwave-assisted distillation (MAD) and microwave hydrodiffusion and gravity (MHG), on the chemical composition of volatile components present in the essential oil and extract of garden nasturtium (<i>T. majus</i> L. <i>var. altum</i>) seeds. Investigation of the biological activity of samples (essential oil, extract and pure compounds) was focused on the antiproliferative effect against different cancer cell lines: cervical cancer cell line (HeLa), human colon cancer cell line (HCT116) and human osteosarcoma cell line (U2OS), and the antibacterial activity which was evaluated against the growth and adhesion of <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> to polystyrene surface.</p><p><strong>Results and conclusions: </strong>Essential oil and extract of garden nasturtium (<i>T. majus</i>) seeds were isolated by two extraction techniques: MAD and MHG. BITC and benzyl cyanide (BCN) present in the extract were identified by gas chromatography-mass spectrometry. Essential oil of <i>T. majus</i> showed higher antiproliferative activity (IC<sub>50</sub><5 µg/mL) than <i>T. majus</i> extract (IC<sub>50</sub><27 µg/mL) against three cancer cell lines: HeLa, HCT116 and U2OS. BITC showed much higher inhibitory effect on all tested cells than BCN. The essential oil and extract of <i>T. majus</i> showed strong antimicrobial activity against <i>S. aureus</i> and <i>E. coli</i>.</p><p><strong>Novelty and scientific contribution: </strong>This work represents the first comparative report on the antiproliferative activity of the essential oil and extract of <i>T. majus</i> seeds, BITC and BCN against HeLa, HCT116 and U2OS cells as well as their antimicrobial activity against <i>S. aureus</i> and <i>E. coli</i>. This study demonstrates that the essential oil of <i>T. majus</i> seeds exhibits stronger antiproliferative and antimicrobial activity than the plant extract.</p>\",\"PeriodicalId\":12400,\"journal\":{\"name\":\"Food Technology and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9901342/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Technology and Biotechnology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.17113/ftb.60.04.22.7667\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Technology and Biotechnology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.17113/ftb.60.04.22.7667","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
研究背景：金莲花（Tropaeolum majus L.）属于金莲花科，含有葡萄糖苷酸苄酯。葡萄糖苷酸苄酯的分解产物异硫氰酸苄酯（BITC）具有多种生物活性，如抗增殖、抗菌和抗炎。为了优化植物提取物和精油中具有生物活性的挥发性化合物的含量，使用适当的提取技术具有至关重要的作用：本研究探讨了微波辅助蒸馏法（MAD）和微波水扩散重力法（MHG）这两种现代萃取方法对金莲花（T. majus L. var. altum）种子精油和萃取物中挥发性成分化学成分的影响。对样品（精油、提取物和纯化合物）生物活性的研究主要集中在对不同癌细胞系（宫颈癌细胞系（HeLa）、人结肠癌细胞系（HCT116）和人骨肉瘤细胞系（U2OS））的抗增殖作用，以及对金黄色葡萄球菌和大肠杆菌在聚苯乙烯表面生长和粘附的抗菌活性的评估：通过两种提取技术分离出了金莲花种子的精油和提取物：MAD 和 MHG。提取物中的 BITC 和苄基氰化物（BCN）通过气相色谱-质谱法进行了鉴定。雄花精油对金黄色葡萄球菌和大肠杆菌具有较高的抗增殖活性（IC50）：本研究首次比较报告了 T. majus 种子、BITC 和 BCN 的精油和提取物对 HeLa、HCT116 和 U2OS 细胞的抗增殖活性以及对金黄色葡萄球菌和大肠杆菌的抗菌活性。这项研究表明，T. majus 种子的精油比植物提取物具有更强的抗增殖和抗菌活性。
Chemical Composition and Biological Activity of Essential Oil and Extract from the Seeds of Tropaeolum majus L. var. altum.
Research background: Plant Tropaeolum majus L. (garden nasturtium) belongs to the family Tropaeolaceae and contains benzyl glucosinolate. The breakdown product of benzyl glucosinolate, benzyl isothiocyanate (BITC), exhibits various biological activities such as antiproliferative, antibacterial and antiinflammatory. In order to optimize the content of biologically active volatile compounds in plant extract and essential oil, the use of appropriate extraction technique has a crucial role.
Experimental approach: The current study investigates the effect of two modern extraction methods, microwave-assisted distillation (MAD) and microwave hydrodiffusion and gravity (MHG), on the chemical composition of volatile components present in the essential oil and extract of garden nasturtium (T. majus L. var. altum) seeds. Investigation of the biological activity of samples (essential oil, extract and pure compounds) was focused on the antiproliferative effect against different cancer cell lines: cervical cancer cell line (HeLa), human colon cancer cell line (HCT116) and human osteosarcoma cell line (U2OS), and the antibacterial activity which was evaluated against the growth and adhesion of Staphylococcus aureus and Escherichia coli to polystyrene surface.
Results and conclusions: Essential oil and extract of garden nasturtium (T. majus) seeds were isolated by two extraction techniques: MAD and MHG. BITC and benzyl cyanide (BCN) present in the extract were identified by gas chromatography-mass spectrometry. Essential oil of T. majus showed higher antiproliferative activity (IC50<5 µg/mL) than T. majus extract (IC50<27 µg/mL) against three cancer cell lines: HeLa, HCT116 and U2OS. BITC showed much higher inhibitory effect on all tested cells than BCN. The essential oil and extract of T. majus showed strong antimicrobial activity against S. aureus and E. coli.
Novelty and scientific contribution: This work represents the first comparative report on the antiproliferative activity of the essential oil and extract of T. majus seeds, BITC and BCN against HeLa, HCT116 and U2OS cells as well as their antimicrobial activity against S. aureus and E. coli. This study demonstrates that the essential oil of T. majus seeds exhibits stronger antiproliferative and antimicrobial activity than the plant extract.
期刊介绍：
Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment.
The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
