中等嗜盐细菌salexigens嗜盐杆菌3EQS1产胞外多糖的结构表征和理化性质。

IF 2.6 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ibrahim M Ibrahim, Yuliya P Fedonenko, Elena N Sigida, Maxim S Kokoulin, Vyacheslav S Grinev, Ivan G Mokrushin, Gennady L Burygin, Andrey M Zakharevich, Alexander A Shirokov, Svetlana A Konnova
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引用次数: 1

摘要

一株菌株3EQS1从埃及法尤姆省卡润湖盐样中分离得到。根据生理生化和系统发育分析,该菌株被归类为salexigens色盐杆菌。菌株3EQS1在含10%蔗糖和10% NaCl的液体矿物培养基(初始pH 8.0)中,在25℃条件下生长72 h,产生了大量(15.1 g L-1)的胞外多糖(EPS)。EPS用冷冻乙醇从无细胞培养基中沉淀,用凝胶渗透和阴离子交换色谱法纯化。EPS的分子量为0.9 × 106 Da。化学分析、傅里叶变换红外光谱和核磁共振谱分析表明,EPS是一个线性β-D-(2→6)链果聚糖(levan)。在水溶液中,EPS倾向于形成超分子聚集体,临界聚集体浓度为240µg mL-1。EPS对煤油(31.2±0.4%)、葵花籽油(76.9±1.3%)和原油(98.9±0.8%)具有较高的乳化活性(E24, %),并具有表面活性剂的性质。0.1% (w/v)的EPS水溶液使水的表面张力降低了11.9%。salexigens 3EQS1的levan可能在各种生物技术过程中有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structural characterization and physicochemical properties of the exopolysaccharide produced by the moderately halophilic bacterium Chromohalobacter salexigens, strain 3EQS1.

Structural characterization and physicochemical properties of the exopolysaccharide produced by the moderately halophilic bacterium Chromohalobacter salexigens, strain 3EQS1.

A strain, 3EQS1, was isolated from a salt sample taken from Lake Qarun (Fayoum Province, Egypt). On the basis of physiological, biochemical, and phylogenetic analyses, the strain was classified as Chromohalobacter salexigens. By 72 h of growth at 25 °C, strain 3EQS1 produced large amounts (15.1 g L-1) of exopolysaccharide (EPS) in a liquid mineral medium (initial pH 8.0) containing 10% sucrose and 10% NaCl. The EPS was precipitated from the cell-free culture medium with chilled ethanol and was purified by gel-permeation and anion-exchange chromatography. The molecular mass of the EPS was 0.9 × 106 Da. Chemical analyses, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy showed that the EPS was a linear β-D-(2 → 6)-linked fructan (levan). In aqueous solution, the EPS tended to form supramolecular aggregates with a critical aggregation concentration of 240 µg mL-1. The EPS had high emulsifying activity (E24, %) against kerosene (31.2 ± 0.4%), sunflower oil (76.9 ± 1.3%), and crude oil (98.9 ± 0.8%), and it also had surfactant properties. A 0.1% (w/v) aqueous EPS solution reduced the surface tension of water by 11.9%. The levan of C. salexigens 3EQS1 may be useful in various biotechnological processes.

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来源期刊
Extremophiles
Extremophiles 生物-生化与分子生物学
CiteScore
6.80
自引率
6.90%
发文量
28
审稿时长
2 months
期刊介绍: Extremophiles features original research articles, reviews, and method papers on the biology, molecular biology, structure, function, and applications of microbial life at high or low temperature, pressure, acidity, alkalinity, salinity, or desiccation; or in the presence of organic solvents, heavy metals, normally toxic substances, or radiation.
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