{"title":"马祖里原螺旋藻的重新发现及其对形态学和多基因系统发育的新贡献(Raabe,1968)Aescht,2001(Ciliophora:Pleuronematida),河蚌Theodoxus fluviatilis Linnaeus的专性共生体,1758(软体动物:腹足目)。","authors":"Tengyue Zhang, Peter Vďačný","doi":"10.1016/j.ejop.2023.125956","DOIUrl":null,"url":null,"abstract":"<div><p>Although the river nerite <em>Theodoxus fluviatilis</em><span> (Gastropoda: Neritimorpha) has an exceptionally broad geographical and ecological distribution, it carries only four ciliate species: </span><em>Hypocomella quatuor</em>, <em>Protospirella mazurica</em>, <em>Scyphidia</em> sp., and <span><em>Trichodina</em><em> baltica</em></span>. After more than a half-century gap, we re-discovered <em>P. mazurica</em> in a Danubian population of <em>T. fluviatilis</em> (haplotypes F31 and F34) and characterized it using an integrative morpho-molecular approach. <em>Protospirella mazurica</em><span> is distinguished by (i) a small, elongate-ellipsoidal to ovoidal body, (ii) a broadly ellipsoidal macronucleus<span><span> accompanied by a single globular micronucleus<span>, (iii) a subterminal contractile vacuole, (iv) about 24 somatic kineties, (v) thigmotactic ciliature composed of about 10 kineties shortened posteriorly to form a parenthetical system, and (vi) a long inverted J-shaped paroral membrane associated with three unequally long membranelles. According to the present </span></span>phylogenetic analyses of two mitochondrial and three nuclear markers, </span></span><em>P. mazurica</em> robustly clusters within the order Pleuronematida (Oligohymenophorea: Scuticociliatia) along with other symbiotic members of the families Hemispeiridae and Thigmophryidae as well as free-living representatives of the paraphyletic family Cyclidiidae. In light of the present phylogenetic analyses, we consider the family Ancistridae to be a junior synonym of the family Hemispeiridae, which collates 14 genera in our classification framework.</p></div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Re-discovery and novel contributions to morphology and multigene phylogeny of Protospirella mazurica (Raabe, 1968) Aescht, 2001 (Ciliophora: Pleuronematida), an obligate symbiont of the river nerite Theodoxus fluviatilis Linnaeus, 1758 (Mollusca: Gastropoda)\",\"authors\":\"Tengyue Zhang, Peter Vďačný\",\"doi\":\"10.1016/j.ejop.2023.125956\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Although the river nerite <em>Theodoxus fluviatilis</em><span> (Gastropoda: Neritimorpha) has an exceptionally broad geographical and ecological distribution, it carries only four ciliate species: </span><em>Hypocomella quatuor</em>, <em>Protospirella mazurica</em>, <em>Scyphidia</em> sp., and <span><em>Trichodina</em><em> baltica</em></span>. After more than a half-century gap, we re-discovered <em>P. mazurica</em> in a Danubian population of <em>T. fluviatilis</em> (haplotypes F31 and F34) and characterized it using an integrative morpho-molecular approach. <em>Protospirella mazurica</em><span> is distinguished by (i) a small, elongate-ellipsoidal to ovoidal body, (ii) a broadly ellipsoidal macronucleus<span><span> accompanied by a single globular micronucleus<span>, (iii) a subterminal contractile vacuole, (iv) about 24 somatic kineties, (v) thigmotactic ciliature composed of about 10 kineties shortened posteriorly to form a parenthetical system, and (vi) a long inverted J-shaped paroral membrane associated with three unequally long membranelles. According to the present </span></span>phylogenetic analyses of two mitochondrial and three nuclear markers, </span></span><em>P. mazurica</em> robustly clusters within the order Pleuronematida (Oligohymenophorea: Scuticociliatia) along with other symbiotic members of the families Hemispeiridae and Thigmophryidae as well as free-living representatives of the paraphyletic family Cyclidiidae. In light of the present phylogenetic analyses, we consider the family Ancistridae to be a junior synonym of the family Hemispeiridae, which collates 14 genera in our classification framework.</p></div>\",\"PeriodicalId\":12042,\"journal\":{\"name\":\"European journal of protistology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European journal of protistology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0932473923000019\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European journal of protistology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0932473923000019","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Re-discovery and novel contributions to morphology and multigene phylogeny of Protospirella mazurica (Raabe, 1968) Aescht, 2001 (Ciliophora: Pleuronematida), an obligate symbiont of the river nerite Theodoxus fluviatilis Linnaeus, 1758 (Mollusca: Gastropoda)
Although the river nerite Theodoxus fluviatilis (Gastropoda: Neritimorpha) has an exceptionally broad geographical and ecological distribution, it carries only four ciliate species: Hypocomella quatuor, Protospirella mazurica, Scyphidia sp., and Trichodina baltica. After more than a half-century gap, we re-discovered P. mazurica in a Danubian population of T. fluviatilis (haplotypes F31 and F34) and characterized it using an integrative morpho-molecular approach. Protospirella mazurica is distinguished by (i) a small, elongate-ellipsoidal to ovoidal body, (ii) a broadly ellipsoidal macronucleus accompanied by a single globular micronucleus, (iii) a subterminal contractile vacuole, (iv) about 24 somatic kineties, (v) thigmotactic ciliature composed of about 10 kineties shortened posteriorly to form a parenthetical system, and (vi) a long inverted J-shaped paroral membrane associated with three unequally long membranelles. According to the present phylogenetic analyses of two mitochondrial and three nuclear markers, P. mazurica robustly clusters within the order Pleuronematida (Oligohymenophorea: Scuticociliatia) along with other symbiotic members of the families Hemispeiridae and Thigmophryidae as well as free-living representatives of the paraphyletic family Cyclidiidae. In light of the present phylogenetic analyses, we consider the family Ancistridae to be a junior synonym of the family Hemispeiridae, which collates 14 genera in our classification framework.
期刊介绍:
Articles deal with protists, unicellular organisms encountered free-living in various habitats or as parasites or used in basic research or applications. The European Journal of Protistology covers topics such as the structure and systematics of protists, their development, ecology, molecular biology and physiology. Beside publishing original articles the journal offers a forum for announcing scientific meetings. Reviews of recently published books are included as well. With its diversity of topics, the European Journal of Protistology is an essential source of information for every active protistologist and for biologists of various fields.