洪都拉斯某三级医院临床标本中分离的念珠菌种类:耳念珠菌在哪里?

Q3 Medicine
Bryan Ortiz, Kateryn Aguilar, Celeste Galindo, Lizzy Molina, Gustavo Fontecha
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引用次数: 0

摘要

背景和目的:新出现的多重耐药念珠菌感染在世界范围内变得越来越频繁。本研究旨在描述念珠菌种类在洪都拉斯三级医院的不同病房。材料和方法:利用分子方法估计白色念珠菌复合体内的物种流行率,利用酵母池DNA提取方法研究金黄色念珠菌。利用表型方法共鉴定出328株酵母菌分离株。为了鉴定白色念珠菌复合体内的物种,采用了基于hpw1基因大小多态性的分子方法。此外,优化了一种基于池中DNA提取的快速鉴定金黄色葡萄球菌的技术。结果:医院病房共检出念珠菌11种。白色念珠菌最多(52.4%);在白色念珠菌复合体中,严感白色念珠菌最为常见,其次是都柏林念珠菌。但未发现C. auris。结论:念珠菌在洪都拉斯的分布报道有限;因此,本研究的数据对于更好地了解其流行病学具有重要意义。此外,还提供了一种简便的金黄色葡萄球菌检测方法,可用于资源匮乏地区的检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

<i>Candida</i> species isolated from clinical samples in a tertiary hospital in Honduras: Where is <i>Candida auris</i>?

<i>Candida</i> species isolated from clinical samples in a tertiary hospital in Honduras: Where is <i>Candida auris</i>?

<i>Candida</i> species isolated from clinical samples in a tertiary hospital in Honduras: Where is <i>Candida auris</i>?

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Background and purpose: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras.

Materials and methods: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris.

Results: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found.

Conclusion: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings.

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来源期刊
Current Medical Mycology
Current Medical Mycology Medicine-Infectious Diseases
CiteScore
2.10
自引率
0.00%
发文量
16
审稿时长
4 weeks
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