{"title":"洪都拉斯某三级医院临床标本中分离的念珠菌种类:耳念珠菌在哪里?","authors":"Bryan Ortiz, Kateryn Aguilar, Celeste Galindo, Lizzy Molina, Gustavo Fontecha","doi":"10.18502/cmm.8.3.11212","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and purpose: </strong>Infections by emerging and multiresistant <i>Candida</i> species are becoming more frequent throughout the world. This study aimed to describe <i>Candida</i> species in different wards of a tertiary hospital in Honduras.</p><p><strong>Materials and methods: </strong>The prevalence of species within the <i>C. albicans</i> complex was estimated using a molecular approach, and <i>C. auris</i> was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the <i>C. albicans</i> complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of <i>C. auris</i>.</p><p><strong>Results: </strong>A total of 11 species of <i>Candida</i> were identified in the hospital wards. <i>C. albicans</i> showed the highest number of isolates (52.4%). Within the <i>C. albicans</i> complex, <i>C. albicans</i> sensu stricto was the most common, followed by <i>C. dubliniensis</i>. However, <i>C. auris</i> was not found.</p><p><strong>Conclusion: </strong>Reports on the distribution of <i>Candida</i> species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of <i>C. auris</i> that could help in its detection in low-resource settings.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"8 3","pages":"1-8"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Candida</i> species isolated from clinical samples in a tertiary hospital in Honduras: Where is <i>Candida auris</i>?\",\"authors\":\"Bryan Ortiz, Kateryn Aguilar, Celeste Galindo, Lizzy Molina, Gustavo Fontecha\",\"doi\":\"10.18502/cmm.8.3.11212\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and purpose: </strong>Infections by emerging and multiresistant <i>Candida</i> species are becoming more frequent throughout the world. This study aimed to describe <i>Candida</i> species in different wards of a tertiary hospital in Honduras.</p><p><strong>Materials and methods: </strong>The prevalence of species within the <i>C. albicans</i> complex was estimated using a molecular approach, and <i>C. auris</i> was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the <i>C. albicans</i> complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of <i>C. auris</i>.</p><p><strong>Results: </strong>A total of 11 species of <i>Candida</i> were identified in the hospital wards. <i>C. albicans</i> showed the highest number of isolates (52.4%). Within the <i>C. albicans</i> complex, <i>C. albicans</i> sensu stricto was the most common, followed by <i>C. dubliniensis</i>. However, <i>C. auris</i> was not found.</p><p><strong>Conclusion: </strong>Reports on the distribution of <i>Candida</i> species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of <i>C. auris</i> that could help in its detection in low-resource settings.</p>\",\"PeriodicalId\":10863,\"journal\":{\"name\":\"Current Medical Mycology\",\"volume\":\"8 3\",\"pages\":\"1-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084484/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Medical Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/cmm.8.3.11212\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Medical Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/cmm.8.3.11212","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?
Background and purpose: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras.
Materials and methods: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast pool-based DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the C. albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris.
Results: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found.
Conclusion: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings.