Humaira Farooq, Tahmina Monowar, Suresh V Chinni, Swe Swe Latt, Noor Hasliza Zainol, Gokul Shankar Sabesan
{"title":"马来西亚混合酵母菌分离株的流行病学和分子鉴定:前进的道路。","authors":"Humaira Farooq, Tahmina Monowar, Suresh V Chinni, Swe Swe Latt, Noor Hasliza Zainol, Gokul Shankar Sabesan","doi":"10.18502/cmm.8.3.11209","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and purpose: </strong>Invasive candidiasis is one of the most common systemic mycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeast culture data.</p><p><strong>Materials and methods: </strong>Yeast isolates were collected in Sultan Abdul Halim Hospital, North Malaysia, from October 2020 to October 2021. Chromogenic <i>Candida</i> differential agar media and PCR-RFLP were used to identify yeast species.</p><p><strong>Results: </strong>A total of 206 yeast isolates were collected from different body sites of patients. The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of <i>Candida</i> species.</p><p><strong>Conclusion: </strong>Malaysia lacks accurate epidemiological data on mixed yeast infections. We identified all samples to the species level, including mixed yeast cultures, using the <i>MspI</i> enzyme and PCR-RFLP.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"8 3","pages":"35-38"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084489/pdf/","citationCount":"0","resultStr":"{\"title\":\"Epidemiology and molecular identification of mixed yeast isolates in Malaysia: A way forward.\",\"authors\":\"Humaira Farooq, Tahmina Monowar, Suresh V Chinni, Swe Swe Latt, Noor Hasliza Zainol, Gokul Shankar Sabesan\",\"doi\":\"10.18502/cmm.8.3.11209\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and purpose: </strong>Invasive candidiasis is one of the most common systemic mycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeast culture data.</p><p><strong>Materials and methods: </strong>Yeast isolates were collected in Sultan Abdul Halim Hospital, North Malaysia, from October 2020 to October 2021. Chromogenic <i>Candida</i> differential agar media and PCR-RFLP were used to identify yeast species.</p><p><strong>Results: </strong>A total of 206 yeast isolates were collected from different body sites of patients. The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of <i>Candida</i> species.</p><p><strong>Conclusion: </strong>Malaysia lacks accurate epidemiological data on mixed yeast infections. We identified all samples to the species level, including mixed yeast cultures, using the <i>MspI</i> enzyme and PCR-RFLP.</p>\",\"PeriodicalId\":10863,\"journal\":{\"name\":\"Current Medical Mycology\",\"volume\":\"8 3\",\"pages\":\"35-38\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084489/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Medical Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/cmm.8.3.11209\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Medical Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/cmm.8.3.11209","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Epidemiology and molecular identification of mixed yeast isolates in Malaysia: A way forward.
Background and purpose: Invasive candidiasis is one of the most common systemic mycoses, and studies have shown mixed yeast infections. Malaysia lacks mixed yeast culture data.
Materials and methods: Yeast isolates were collected in Sultan Abdul Halim Hospital, North Malaysia, from October 2020 to October 2021. Chromogenic Candida differential agar media and PCR-RFLP were used to identify yeast species.
Results: A total of 206 yeast isolates were collected from different body sites of patients. The majority of the yeast isolates (n=104) were obtained from the urine. Other isolates were extracted from blood (n=52), vaginal swabs (n=45), ear discharge (n=2), tracheal aspirate (n=2), tissue (n=2), skin (n=1), nail (n=1), sputum (n=1), and cerebrospinal fluid (n=1). In total, 200 yeast samples were identified as single species, and six isolates were a mixture of Candida species.
Conclusion: Malaysia lacks accurate epidemiological data on mixed yeast infections. We identified all samples to the species level, including mixed yeast cultures, using the MspI enzyme and PCR-RFLP.
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