短期饲粮添加对日本黑牛卵巢卵泡数量、卵母细胞数量和质量及体外胚胎产生的影响。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Kensuke Tomita, Toshimichi Ishii, Natsumi Endo, Tomomi Tanaka
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引用次数: 1

摘要

本研究旨在验证短期补充高能日粮促进日本黑牛取卵后胚胎产生的假设。经过一段时间的维持饮食适应后,高能量饮食组(HD组,n = 6)饲喂200%维持饮食,另一组(MD组,n = 6)饲喂维持饮食,持续4周。于第14、21、28天进行opu体外受精(IVF)手术;对卵泡和卵母细胞进行计数和形态学分级,培养可培养的卵母细胞进行体外成熟、受精和培养。HD组第14、21天的平均血浆胰岛素浓度显著高于MD组(P < 0.05)。HD组在OPU时观察到的卵泡数、恢复卵母细胞数、可培养(1 ~ 4级)卵母细胞数和退化(6级)卵母细胞率均显著高于MD组(P < 0.05)。HD组卵裂卵母细胞比例低于MD组(P < 0.05);因此,在HD组和MD组之间获得的囊胚数量没有显著差异。目前的研究结果表明,高能日粮可以促进卵泡生长,同时增加血浆胰岛素浓度,但对日本黑牛OPU-IVF手术的卵母细胞质量有不利影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and in vitro embryo production in Japanese Black cows.

Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and in vitro embryo production in Japanese Black cows.

Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and in vitro embryo production in Japanese Black cows.

Effects of short-term dietary supplementation on the number of ovarian follicles, quantity and quality of oocytes, and in vitro embryo production in Japanese Black cows.

This study aimed to test the hypothesis that short-term supplementation with a high-energy diet promotes embryo production following ovum pick-up (OPU) in Japanese Black cows. After a period of adaptation to the maintenance diet, a 200% maintenance diet was fed to the high-energy diet group (HD group, n = 6) for four weeks, and a maintenance diet was fed to the other group (MD group, n = 6). OPU-in vitro fertilization (IVF) procedures were performed on days 14, 21, and 28; follicles and oocytes were counted and morphologically graded, and cultivable oocytes were cultured for in vitro maturation, fertilization, and culture. The mean plasma insulin concentrations on days 14 and 21 were significantly higher in the HD group than in the MD group (P < 0.05). The number of follicles observed at OPU, recovered oocytes, cultivable (Grades 1 to 4) oocytes, and the rate of degenerated (Grade 6) oocytes in the HD group were significantly higher than those in the MD group (P < 0.05). The proportion of cleaved oocytes was lower in the HD group than in the MD group (P < 0.05); consequently, there was no significant difference in the number of blastocysts obtained between the HD and MD groups. The present findings suggest that high-energy diets can promote follicular growth in parallel with an increase in plasma concentrations of insulin, but have a detrimental effect on the quality of oocytes with the OPU-IVF procedure in Japanese Black cows.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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