Daiana de Souza Perce-da-Silva, Thays Euzebio Joaquim, Ana Luisa Quintella do Couto Aleixo, Juliana Pessanha Rodrigues Motta, Josué da Costa Lima-Junior, Marcelo Ribeiro-Alves, Joseli de Oliveira-Ferreira, Luís Cristóvão de Moraes Sobrino Porto, Dalma Maria Banic, Maria Regina Reis Amendoeira
{"title":"杀伤免疫球蛋白样受体基因对巴西眼弓形虫病复发率的影响。","authors":"Daiana de Souza Perce-da-Silva, Thays Euzebio Joaquim, Ana Luisa Quintella do Couto Aleixo, Juliana Pessanha Rodrigues Motta, Josué da Costa Lima-Junior, Marcelo Ribeiro-Alves, Joseli de Oliveira-Ferreira, Luís Cristóvão de Moraes Sobrino Porto, Dalma Maria Banic, Maria Regina Reis Amendoeira","doi":"10.1590/0074-02760220203","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Recurrence is a hallmark of ocular toxoplasmosis (OT), and conditions that influence its occurrence remain a challenge. Natural killer cells (NK) are effectors cells whose primary is cytotoxic function against many parasites, including Toxoplasma gondii. Among the NK cell receptors, immunoglobulin-like receptors (KIR) deserve attention due to their high polymorphism.</p><p><strong>Objectives: </strong>This study aimed to analyse the influence of KIR gene polymorphism in the course of OT infection and its association with recurrences after an active episode.</p><p><strong>Methods: </strong>Ninety-six patients from the Ophthalmologic Clinic of the National Institute of Infectology Evandro Chagas were followed for up to five years. After DNA extraction, genotyping of the patients was performed by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) utilising Luminex equipment for reading. During follow-up, 60.4% had a recurrence.</p><p><strong>Findings: </strong>We identified 25 KIR genotypes and found a higher frequency of genotype 1 (31.7%) with worldwide distribution. We note that the KIR2DL2 inhibitor gene and the gene activator KIR2DS2 were more frequent in patients without recurrence. Additionally, we observed that individuals who carry these genes progressed recurrence episodes slowly compared to individuals who do not carry these genes.</p><p><strong>Main conclusions: </strong>The KIR2DL2 and KIR2DS2 are associated as possible protection markers against ocular toxoplasmosis recurrence (OTR).</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220203"},"PeriodicalIF":2.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10065411/pdf/","citationCount":"1","resultStr":"{\"title\":\"Influence of killer immunoglobulin-like receptors genes on the recurrence rate of ocular toxoplasmosis in Brazil.\",\"authors\":\"Daiana de Souza Perce-da-Silva, Thays Euzebio Joaquim, Ana Luisa Quintella do Couto Aleixo, Juliana Pessanha Rodrigues Motta, Josué da Costa Lima-Junior, Marcelo Ribeiro-Alves, Joseli de Oliveira-Ferreira, Luís Cristóvão de Moraes Sobrino Porto, Dalma Maria Banic, Maria Regina Reis Amendoeira\",\"doi\":\"10.1590/0074-02760220203\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Recurrence is a hallmark of ocular toxoplasmosis (OT), and conditions that influence its occurrence remain a challenge. 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Influence of killer immunoglobulin-like receptors genes on the recurrence rate of ocular toxoplasmosis in Brazil.
Background: Recurrence is a hallmark of ocular toxoplasmosis (OT), and conditions that influence its occurrence remain a challenge. Natural killer cells (NK) are effectors cells whose primary is cytotoxic function against many parasites, including Toxoplasma gondii. Among the NK cell receptors, immunoglobulin-like receptors (KIR) deserve attention due to their high polymorphism.
Objectives: This study aimed to analyse the influence of KIR gene polymorphism in the course of OT infection and its association with recurrences after an active episode.
Methods: Ninety-six patients from the Ophthalmologic Clinic of the National Institute of Infectology Evandro Chagas were followed for up to five years. After DNA extraction, genotyping of the patients was performed by polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) utilising Luminex equipment for reading. During follow-up, 60.4% had a recurrence.
Findings: We identified 25 KIR genotypes and found a higher frequency of genotype 1 (31.7%) with worldwide distribution. We note that the KIR2DL2 inhibitor gene and the gene activator KIR2DS2 were more frequent in patients without recurrence. Additionally, we observed that individuals who carry these genes progressed recurrence episodes slowly compared to individuals who do not carry these genes.
Main conclusions: The KIR2DL2 and KIR2DS2 are associated as possible protection markers against ocular toxoplasmosis recurrence (OTR).
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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