Sandrine Le Gall-David, Gaëlle Boudry, Sylvie Buffet-Bataillon
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引用次数: 0
摘要
目的:研究4种广泛使用的DNA提取试剂盒对不同类型的高(粪便)和低生物量样本(食糜、支气管肺泡灌洗液和痰液)的提取性能。方法:对Qiagen Powerfecal Pro DNA试剂盒、Macherey Nucleospin Soil试剂盒、Macherey Nucleospin Tissue试剂盒和MagnaPure LC DNA分离试剂盒III进行DNA数量、质量、多样性和组成分析。结果:4种试剂盒中DNA的数量和质量存在差异。4个试剂盒粪便样品的微生物群多样性和组成特征相似。结论:虽然4种试剂盒的DNA质量和数量不同，但对粪便样品的检测结果相似，但对低生物量样品的检测灵敏度均不够高。
Comparison of four DNA extraction kits efficiency for 16SrDNA microbiota profiling of diverse human samples.
Aim: The current study investigated the performance of 4 widely used DNA extraction kits using different types of high (stool) and low biomass samples (chyme, broncho alveolar lavage and sputum).
Methods: Qiagen Powerfecal Pro DNA kit, Macherey Nucleospin Soil kit, Macherey Nucleospin Tissue Kit and MagnaPure LC DNA isolation kit III were evaluated in terms of DNA quantity, quality, diversity and composition profiles.
Results: The quantity and quality of DNA varied among the four kits. The microbiota of the stool samples showed similar diversity and compositional profiles for the 4 kits.
Conclusion: Despite differences in DNA quality and quantity, the 4 kits yielded similar results for stool samples, while all kits were not sensitive enough for low biomass samples.
期刊介绍：
Future Science OA is an online, open access, peer-reviewed title from the Future Science Group. The journal covers research and discussion related to advances in biotechnology, medicine and health. The journal embraces the importance of publishing all good-quality research with the potential to further the progress of research in these fields. All original research articles will be considered that are within the journal''s scope, and have been conducted with scientific rigour and research integrity. The journal also features review articles, editorials and perspectives, providing readers with a leading source of commentary and analysis. Submissions of the following article types will be considered: -Research articles -Preliminary communications -Short communications -Methodologies -Trial design articles -Trial results (including early-phase and negative studies) -Reviews -Perspectives -Commentaries
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