mmp -2缺陷小鼠下颌髁I型和II型胶原蛋白、聚集蛋白、MMP-9和MMP-13的免疫组织化学表征

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Mu-Chen Yang, Megumi Nakamura, Miyuki Mayanagi, Yasuyuki Sasano
{"title":"mmp -2缺陷小鼠下颌髁I型和II型胶原蛋白、聚集蛋白、MMP-9和MMP-13的免疫组织化学表征","authors":"Mu-Chen Yang,&nbsp;Megumi Nakamura,&nbsp;Miyuki Mayanagi,&nbsp;Yasuyuki Sasano","doi":"10.2220/biomedres.44.65","DOIUrl":null,"url":null,"abstract":"<p><p>Mice devoid of matrix metalloproteinase (MMP)-2 due to gene targeting have been reported to show articular cartilage destruction in the knee joint; however, the phenotype of the mandibular condylar cartilage remains unknown. Thus, in the present study, we investigated the mandibular condyle in Mmp2<sup>-/-</sup> mice. We obtained and bred Mmp2<sup>-/-</sup> mice from the same source as the previous study, and performed genotyping using genomic DNA extracted from finger snips. The mandibular condyle of Mmp2<sup>-/-</sup> mice and wild-type (WT) mice was immunohistochemically examined for the localization of extracellular matrix (ECM) proteins (type I and II collagen, and aggrecan), and MMP-9 and MMP-13. No cartilage destruction was observed in the mandibular condyle of Mmp2<sup>-/-</sup> mice, and no difference was found in the localization of the ECM proteins between the Mmp2<sup>-/-</sup> mice and WT mice. However, the bone marrow cavity in the subchondral bone of the mandibular condyle was more distinct in Mmp2<sup>-/-</sup> mice than in WT mice at the age of 50 weeks. Of note, MMP-9 characteristically localized in multinucleated cells in the mandibular condyle in 50-week-old Mmp2<sup>-/-</sup> mice. MMP-2 may be involved in the regulation of osteoclast differentiation and the formation of the bone marrow cavity in aged mice.</p>","PeriodicalId":9138,"journal":{"name":"Biomedical Research-tokyo","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Immunohistochemical characterization of the mandibular condyle for type I and II collagen, aggrecan, MMP-9, and MMP-13 in MMP-2-deficient mice.\",\"authors\":\"Mu-Chen Yang,&nbsp;Megumi Nakamura,&nbsp;Miyuki Mayanagi,&nbsp;Yasuyuki Sasano\",\"doi\":\"10.2220/biomedres.44.65\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mice devoid of matrix metalloproteinase (MMP)-2 due to gene targeting have been reported to show articular cartilage destruction in the knee joint; however, the phenotype of the mandibular condylar cartilage remains unknown. Thus, in the present study, we investigated the mandibular condyle in Mmp2<sup>-/-</sup> mice. We obtained and bred Mmp2<sup>-/-</sup> mice from the same source as the previous study, and performed genotyping using genomic DNA extracted from finger snips. The mandibular condyle of Mmp2<sup>-/-</sup> mice and wild-type (WT) mice was immunohistochemically examined for the localization of extracellular matrix (ECM) proteins (type I and II collagen, and aggrecan), and MMP-9 and MMP-13. No cartilage destruction was observed in the mandibular condyle of Mmp2<sup>-/-</sup> mice, and no difference was found in the localization of the ECM proteins between the Mmp2<sup>-/-</sup> mice and WT mice. However, the bone marrow cavity in the subchondral bone of the mandibular condyle was more distinct in Mmp2<sup>-/-</sup> mice than in WT mice at the age of 50 weeks. Of note, MMP-9 characteristically localized in multinucleated cells in the mandibular condyle in 50-week-old Mmp2<sup>-/-</sup> mice. MMP-2 may be involved in the regulation of osteoclast differentiation and the formation of the bone marrow cavity in aged mice.</p>\",\"PeriodicalId\":9138,\"journal\":{\"name\":\"Biomedical Research-tokyo\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Research-tokyo\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2220/biomedres.44.65\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Research-tokyo","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2220/biomedres.44.65","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
引用次数: 0

摘要

据报道,由于基因靶向而缺乏基质金属蛋白酶(MMP)-2的小鼠在膝关节显示关节软骨破坏;然而,下颌髁软骨的表型仍然是未知的。因此,在本研究中,我们研究了Mmp2-/-小鼠的下颌髁。我们从与先前研究相同的来源获得并繁殖了Mmp2-/-小鼠,并使用从手指剪中提取的基因组DNA进行基因分型。免疫组织化学检测Mmp2-/-小鼠和野生型(WT)小鼠下颌髁的细胞外基质(ECM)蛋白(I型和II型胶原蛋白和聚集蛋白)和MMP-9和MMP-13的定位。Mmp2-/-小鼠下颌髁未见软骨破坏,ECM蛋白的定位在Mmp2-/-小鼠和WT小鼠之间无差异。然而,在50周龄时,Mmp2-/-小鼠下颌髁软骨下骨的骨髓腔比WT小鼠更明显。值得注意的是,在50周龄的Mmp2-/-小鼠中,MMP-9特征性地定位于下颌髁的多核细胞中。MMP-2可能参与老龄小鼠破骨细胞分化和骨髓腔形成的调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical characterization of the mandibular condyle for type I and II collagen, aggrecan, MMP-9, and MMP-13 in MMP-2-deficient mice.

Mice devoid of matrix metalloproteinase (MMP)-2 due to gene targeting have been reported to show articular cartilage destruction in the knee joint; however, the phenotype of the mandibular condylar cartilage remains unknown. Thus, in the present study, we investigated the mandibular condyle in Mmp2-/- mice. We obtained and bred Mmp2-/- mice from the same source as the previous study, and performed genotyping using genomic DNA extracted from finger snips. The mandibular condyle of Mmp2-/- mice and wild-type (WT) mice was immunohistochemically examined for the localization of extracellular matrix (ECM) proteins (type I and II collagen, and aggrecan), and MMP-9 and MMP-13. No cartilage destruction was observed in the mandibular condyle of Mmp2-/- mice, and no difference was found in the localization of the ECM proteins between the Mmp2-/- mice and WT mice. However, the bone marrow cavity in the subchondral bone of the mandibular condyle was more distinct in Mmp2-/- mice than in WT mice at the age of 50 weeks. Of note, MMP-9 characteristically localized in multinucleated cells in the mandibular condyle in 50-week-old Mmp2-/- mice. MMP-2 may be involved in the regulation of osteoclast differentiation and the formation of the bone marrow cavity in aged mice.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信