Carolina de O Mendes-Aguiar, Milene Yoko Kitahara-Oliveira, Ana Cristina Oliveira de Almeida, Marcia Pereira-Oliveira, Manoel Paes de Oliveira Neto, Claude Pirmez, Elizabeth Pereira Sampaio, Adriano Gomes-Silva, Alda Maria Da-Cruz
{"title":"皮肤利什曼病病变细胞表达 DC-SIGN 受体，并与巴西利什曼病和亚马逊利什曼病原虫进行不同程度的结合。","authors":"Carolina de O Mendes-Aguiar, Milene Yoko Kitahara-Oliveira, Ana Cristina Oliveira de Almeida, Marcia Pereira-Oliveira, Manoel Paes de Oliveira Neto, Claude Pirmez, Elizabeth Pereira Sampaio, Adriano Gomes-Silva, Alda Maria Da-Cruz","doi":"10.1590/0074-02760220044","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome.</p><p><strong>Objectives: </strong>In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes.</p><p><strong>Methods: </strong>DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture.</p><p><strong>Results: </strong>In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations.</p><p><strong>Conclusion: </strong>Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220044"},"PeriodicalIF":2.5000,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10042235/pdf/","citationCount":"0","resultStr":"{\"title\":\"DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. 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(L.) amazonensis (La) promastigotes.</p><p><strong>Methods: </strong>DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture.</p><p><strong>Results: </strong>In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations.</p><p><strong>Conclusion: </strong>Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. 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引用次数: 0
摘要
背景：树突状细胞（DCs）特异性细胞间粘附分子（ICAM）-3抓取非整合素受体（DC-SIGN）与利什曼原虫亚属结合，介导其与DC和中性粒细胞的相互作用，可能影响感染结果：在这项工作中，我们研究了DC-SIGN受体是否在皮肤利什曼病（CL）病变细胞中表达，以及体外与巴西利什曼原虫（Lb）和亚马逊利什曼原虫（La）的结合模式：方法：用免疫组织化学方法标记冷冻保存的 CL 组织片段中的 DC-SIGN 受体。在共培养 2 h、24 h 和 48 h 时，用流式细胞术监测与 CFSE 标记的 Lb 或 La 原虫和表达 DC-SIGN 的 RAJI 转染细胞（DC-SIGNPOS）或模拟转染细胞（DC-SIGNNEG）的体外结合试验：结果：在 CL 病变浸润区，DC-SIGNPOS 细胞存在于真皮层和表皮层附近。Lb和La都与DC-SIGNPOS细胞结合，而与DC-SIGNNEG的结合率较低。与 DC-SIGNlow 细胞相比，La 与 DC-SIGNhi 细胞的结合更早，亲和力更高，而 Lb 与这些细胞的结合情况相似：结论：我们的研究结果表明，DC-SIGN受体存在于L. Braziliensis CL病变中，并与Lb原虫相互作用。此外，与 Lb 和 La 的结合模式差异表明，DC-SIGN 可以在利什曼原虫感染后的最初几个小时以不同的方式影响寄生虫的摄入。这些结果提出了一个假设，即DC-SIGN受体可能参与美洲皮损利什曼病的免疫发病机制，从而导致利什曼原虫感染结果的差异。
DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes.
Background: Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome.
Objectives: In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes.
Methods: DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture.
Results: In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations.
Conclusion: Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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