Bert Gerrits van den Ende , Anderson Messias Rodrigues , Rosane Christine Hahn , Ferry Hagen
{"title":"一个令人惊讶的发现:舌头病变误诊为副球孢子菌病的奇怪病例","authors":"Bert Gerrits van den Ende , Anderson Messias Rodrigues , Rosane Christine Hahn , Ferry Hagen","doi":"10.1016/j.riam.2022.11.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Paracoccidioidomycosis is an endemic mycosis caused by members of the <em>Paracoccidioides</em> genus. Brazil remains the focus area and, to a lesser extent, the disease has been reported from Argentina, Colombia and Venezuela.</p></div><div><h3>Aims</h3><p>A Venezuelan <em>Paracoccidioides brasiliensis</em> strain, isolated from a patient diagnosed with chronic multifocal paracoccidioidomycosis, was subjected to whole genome sequencing to provide more insight about <em>Paracoccidioides</em> outside the endemic focus area.</p></div><div><h3>Methods</h3><p><em>P. brasiliensis</em> strain CBS 118890 was whole genome sequenced using nanopore; library preparation with the ‘native barcoding genomic DNA kit’ was followed by sequencing on Flongle and MinION flowcells. Batches of strain CBS 118890 were re-identified by sequencing the internal transcribed spacer (ITS) region, and final identification was made based on phylogenetic analysis.</p></div><div><h3>Results</h3><p>Surprisingly, the Venezuelan <em>P. brasiliensis</em> strain CBS 118890 turned out to be a <em>Nannizziopsis</em> species. The batches of this strain were ITS sequenced followed by phylogenetic analysis and resulted in the final identification of <em>Nannizziopsis arthrosporioides</em>.</p></div><div><h3>Conclusions</h3><p><em>Nannizziopsis</em> infections are commonly seen in a wide variety of reptiles, but are particularly rare in human infections. This case underlines the need for molecular characterization of cases that clinically mimic paracoccidioidomycosis but that are serologically negative for <em>Paracoccidioides</em>.</p></div>","PeriodicalId":21291,"journal":{"name":"Revista Iberoamericana De Micologia","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A surprising finding: The curious case of a tongue lesion misdiagnosed as paracoccidioidomycosis\",\"authors\":\"Bert Gerrits van den Ende , Anderson Messias Rodrigues , Rosane Christine Hahn , Ferry Hagen\",\"doi\":\"10.1016/j.riam.2022.11.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Paracoccidioidomycosis is an endemic mycosis caused by members of the <em>Paracoccidioides</em> genus. Brazil remains the focus area and, to a lesser extent, the disease has been reported from Argentina, Colombia and Venezuela.</p></div><div><h3>Aims</h3><p>A Venezuelan <em>Paracoccidioides brasiliensis</em> strain, isolated from a patient diagnosed with chronic multifocal paracoccidioidomycosis, was subjected to whole genome sequencing to provide more insight about <em>Paracoccidioides</em> outside the endemic focus area.</p></div><div><h3>Methods</h3><p><em>P. brasiliensis</em> strain CBS 118890 was whole genome sequenced using nanopore; library preparation with the ‘native barcoding genomic DNA kit’ was followed by sequencing on Flongle and MinION flowcells. Batches of strain CBS 118890 were re-identified by sequencing the internal transcribed spacer (ITS) region, and final identification was made based on phylogenetic analysis.</p></div><div><h3>Results</h3><p>Surprisingly, the Venezuelan <em>P. brasiliensis</em> strain CBS 118890 turned out to be a <em>Nannizziopsis</em> species. The batches of this strain were ITS sequenced followed by phylogenetic analysis and resulted in the final identification of <em>Nannizziopsis arthrosporioides</em>.</p></div><div><h3>Conclusions</h3><p><em>Nannizziopsis</em> infections are commonly seen in a wide variety of reptiles, but are particularly rare in human infections. This case underlines the need for molecular characterization of cases that clinically mimic paracoccidioidomycosis but that are serologically negative for <em>Paracoccidioides</em>.</p></div>\",\"PeriodicalId\":21291,\"journal\":{\"name\":\"Revista Iberoamericana De Micologia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista Iberoamericana De Micologia\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1130140622000389\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista Iberoamericana De Micologia","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1130140622000389","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MYCOLOGY","Score":null,"Total":0}
A surprising finding: The curious case of a tongue lesion misdiagnosed as paracoccidioidomycosis
Background
Paracoccidioidomycosis is an endemic mycosis caused by members of the Paracoccidioides genus. Brazil remains the focus area and, to a lesser extent, the disease has been reported from Argentina, Colombia and Venezuela.
Aims
A Venezuelan Paracoccidioides brasiliensis strain, isolated from a patient diagnosed with chronic multifocal paracoccidioidomycosis, was subjected to whole genome sequencing to provide more insight about Paracoccidioides outside the endemic focus area.
Methods
P. brasiliensis strain CBS 118890 was whole genome sequenced using nanopore; library preparation with the ‘native barcoding genomic DNA kit’ was followed by sequencing on Flongle and MinION flowcells. Batches of strain CBS 118890 were re-identified by sequencing the internal transcribed spacer (ITS) region, and final identification was made based on phylogenetic analysis.
Results
Surprisingly, the Venezuelan P. brasiliensis strain CBS 118890 turned out to be a Nannizziopsis species. The batches of this strain were ITS sequenced followed by phylogenetic analysis and resulted in the final identification of Nannizziopsis arthrosporioides.
Conclusions
Nannizziopsis infections are commonly seen in a wide variety of reptiles, but are particularly rare in human infections. This case underlines the need for molecular characterization of cases that clinically mimic paracoccidioidomycosis but that are serologically negative for Paracoccidioides.
期刊介绍:
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.