甲壳素纳米原纤维包覆细菌纤维素电纺丝网用于耳膜修复。

IF 3.5 3区 医学 Q3 CELL & TISSUE ENGINEERING
Tissue Engineering Part A Pub Date : 2024-04-01 Epub Date: 2024-01-25 DOI:10.1089/ten.TEA.2023.0242
Bahareh Azimi, Atefeh Rasti, Alessandra Fusco, Teresa Macchi, Claudio Ricci, Mohammad Amin Hosseinifard, Lorenzo Guazzelli, Giovanna Donnarumma, Roohollah Bagherzadeh, Masoud Latifi, Ipsita Roy, Serena Danti, Andrea Lazzeri
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引用次数: 0

摘要

在这项研究中,我们开发了一种基于细菌纤维素(BC)和几丁质纳米纤维(CNs)的生物基和生物活性纳米纤维贴片,以离子液体作为BC的溶剂,旨在修复鼓膜(TM)。采用静电纺丝法制备了BC纳米纤维网,并用电喷雾对其表面进行了CNs改性。研究了BC/离子液体体系的流变性。得到的CN/BC网格进行了全面的形态、物理化学和力学表征。使用L929小鼠成纤维细胞进行细胞毒性试验,发现细胞存活率为97.8%。在兔皮肤上进行的体内试验表明,该贴片无刺激性。以人真皮角化细胞(HaCaT细胞)和人脐静脉内皮细胞(HUVECs)为模型细胞,体外测试了CN/BC纤维网对TM穿孔愈合的影响。两种类型的细胞都在这些支架上成功生长。CNs的存在提高了静电纺纤维网的间接抗菌活性。HaCaT细胞在6小时和24小时表现出对伤口愈合过程至关重要的关键促炎细胞因子mRNA表达上调,表明CNs在愈合反应中的潜在益处。总的来说,本研究提出了一种天然和生态可持续的纤维网,在TM修复中有很大的应用前景,利用BC和CNs的协同作用,可能促进组织再生和愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacterial Cellulose Electrospun Fiber Mesh Coated with Chitin Nanofibrils for Eardrum Repair.

In this study, we develop a bio-based and bioactive nanofibrous patch based on bacterial cellulose (BC) and chitin nanofibrils (CNs) using an ionic liquid as a solvent for BC, aimed at tympanic membrane (TM) repair. Electrospun BC nanofiber meshes were produced via electrospinning, and surface-modified with CNs using electrospray. The rheology of the BC/ionic liquid system was investigated. The obtained CN/BC meshes underwent comprehensive morphological, physicochemical, and mechanical characterization. Cytotoxicity tests were conducted using L929 mouse fibroblasts, revealing a cell viability of 97.8%. In vivo tests on rabbit skin demonstrated that the patches were nonirritating. Furthermore, the CN/BC fiber meshes were tested in vitro using human dermal keratinocytes (HaCaT cells) and human umbilical vein endothelial cells as model cells for TM perforation healing. Both cell types demonstrated successful growth on these scaffolds. The presence of CNs resulted in improved indirect antimicrobial activity of the electrospun fiber meshes. HaCaT cells exhibited an upregulated mRNA expression at 6 and 24 h of key proinflammatory cytokines crucial for the wound healing process, indicating the potential benefits of CNs in the healing response. Overall, this study presents a natural and eco-sustainable fiber mesh with great promise for applications in TM repair, leveraging the synergistic effects of BC and CNs to possibly enhance tissue regeneration and healing. Impact statement Repair of tympanic membrane perforations following chronic otitis media is a main clinical issue in otologic surgery, where the underlying infection obstacles self-healing. To address this challenge, our study proposes a bio-based patch made of nanoscale carbohydrate materials (i.e., bacterial cellulose electrospun fibers and chitin nanofibrils) processed via green solvents. The scaffold is nonirritating in vivo, and cytocompatible with fibroblasts, endothelial cells, and keratinocytes. In epithelial cells, it stimulates the expression of the antimicrobial peptide human beta defensin 2, with a pathway of cytokine expression compatible with the wound healing process. Therefore, it could be applied with unsolved infective pathology.

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来源期刊
Tissue Engineering Part A
Tissue Engineering Part A Chemical Engineering-Bioengineering
CiteScore
9.20
自引率
2.40%
发文量
163
审稿时长
3 months
期刊介绍: Tissue Engineering is the preeminent, biomedical journal advancing the field with cutting-edge research and applications that repair or regenerate portions or whole tissues. This multidisciplinary journal brings together the principles of engineering and life sciences in the creation of artificial tissues and regenerative medicine. Tissue Engineering is divided into three parts, providing a central forum for groundbreaking scientific research and developments of clinical applications from leading experts in the field that will enable the functional replacement of tissues.
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