发酵黑参提取物通过调节人角质形成细胞的nc886-PKR通路来预防uvb诱导的炎症。

IF 2.5 4区医学 Q2 DERMATOLOGY

Photodermatology, photoimmunology & photomedicine Pub Date : 2024-01-01 Epub Date: 2023-11-14 DOI:10.1111/phpp.12927

Yuna Kim, Junbo Sim, Kyungeun Jeon, Dehun Ryu, Youngeun Ji, Youngseok Kim, Junoh Kim, Suwon Jeon, Deokhoon Park, Eunsun Jung

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引用次数: 0

摘要

背景:皮肤持续暴露在紫外线B (UVB)射线下会引起炎症和光损伤。在之前的研究中，我们发现上调非编码RNA (ncRNA) nc886可以通过抑制蛋白激酶RNA (PKR)通路来缓解uvb诱导的炎症。我们的目的是研究发酵黑参提取物(FBGE)对uvb诱导的角化细胞炎症的影响，该提取物已被证明可以增加nc886的表达。方法:采用MTT法对人角质形成细胞无明显细胞毒性作用。通过qPCR、Western blotting和ELISA分析评估FBGE的疗效，证实其对uvb诱导炎症的调节作用。结果:分析结果显示，FBGE抑制了UVB引起的nc886表达下降和nc886甲基化的增加。抑制uvb诱导的金属蛋白酶-9 (MMP-9)、金属蛋白酶-1 (MMP-1)、环氧合酶-2 (COX-2)、前列腺素E2 (PGE2)、白细胞介素-8 (IL-8)、肿瘤坏死因子-α (TNF-α)的升高。此外，FBGE抑制了UVB激活的PKR-MAPK通路。结论:FBGE可通过调节nc886-PKR通路减轻uvb诱导的炎症。

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Fermented black ginseng extract prevents UVB-induced inflammation by regulating the nc886-PKR pathway in human keratinocytes.

Background: Continuous exposure of the skin to ultraviolet B (UVB) rays can cause inflammation and photodamage. In previous studies, we observed that the upregulation of nc886, a noncoding RNA (ncRNA), can alleviate UVB-induced inflammation through suppression of the protein kinase RNA (PKR) pathway. We aim to investigate the effect of fermented black ginseng extract (FBGE), which has been shown to increase the expression of nc886, on UVB-induced inflammation in keratinocytes.

Methods: To confirm the cytotoxicity of FBGE, MTT assay was performed, and no significant cytotoxicity was found on human keratinocytes. The efficacies of FBGE were assessed through qPCR, Western blotting, and ELISA analysis which confirmed regulation of UVB-induced inflammation.

Results: The analysis results showed that FBGE inhibited the decrease in nc886 expression and the increase in the methylated nc886 caused by UVB. It also prevented the UVB-induced increase of metalloproteinase-9 (MMP-9), metalloproteinase-1 (MMP-1), cyclooxygenase-2 (COX-2), prostaglandin E₂ (PGE2), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α). Additionally, FBGE suppressed the PKR-MAPK pathways activated by UVB.

Conclusion: These results implicate that FBGE can alleviate UVB-induced inflammation through regulation of the nc886-PKR pathway.

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来源期刊

Photodermatology, photoimmunology & photomedicine 医学-皮肤病学

CiteScore

4.40

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal is a forum for new information about the direct and distant effects of electromagnetic radiation (ultraviolet, visible and infrared) mediated through skin. The divisions of the editorial board reflect areas of specific interest: aging, carcinogenesis, immunology, instrumentation and optics, lasers, photodynamic therapy, photosensitivity, pigmentation and therapy. Photodermatology, Photoimmunology & Photomedicine includes original articles, reviews, communications and editorials. Original articles may include the investigation of experimental or pathological processes in humans or animals in vivo or the investigation of radiation effects in cells or tissues in vitro. Methodology need have no limitation; rather, it should be appropriate to the question addressed.