二倍体和三倍体太平洋鲍鱼的细胞遗传学比较分析。

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Cytogenetic and Genome Research Pub Date : 2023-01-01 Epub Date: 2023-11-13 DOI:10.1159/000535045
Jianpeng Zhang, Yi Wang, Ying Lu, Weiwei You, Xuan Luo, Caihuan Ke
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引用次数: 0

摘要

对化学处理后的三倍体和二倍体盘蝇幼虫进行了细胞遗传学分析。结果表明,三倍体铁饼染色体数目为3n = 54,由30个稳中心(m)和24个亚稳中心(sm)染色体组成;二倍体染色体数目为2n = 36,由20个稳中心(m)和16个亚稳中心(sm)染色体组成。值得注意的是,三倍体和二倍体在NORs数量和/或直径上都表现出差异。三倍体NORs的平均数量显著高于二倍体(P < 0.05),两组NORs的平均直径差异不显著(P < 0.05)。此外,在三倍体中观察到5S rDNA定位在3个亚着丝中心染色体上,而在二倍体中观察到2个亚着丝中心染色体。18S rDNA位点在二倍体和三倍体中均表现出位置保护和数量变异。其中,18S rDNA位于染色体末端,三倍体的位点数量显著高于二倍体(P < 0.01)。本研究为进一步了解三倍体的细胞遗传学特征提供了有价值的见解,为进一步研究该物种染色体组的稳定性提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Cytogenetic Analysis of Diploid and Triploid Pacific Abalone, Haliotis discus hannai.

Introduction: The Pacific abalone, Haliotis discus hannai, is one of the most commercially important marine shellfish in China. Cell engineering breeding is an important tool in abalone genetic breeding, and the triploids obtained through this method have high commercial value. However, current research mainly focuses on establishing induction methods and evaluating the growth traits of triploids, while there is a lack of basic research on triploid cytogenetics.

Method: In this study, Cytogenetic analysis of triploid Haliotis discus hannai larvae (produced by chemical treatment) and diploid larvae was performed.

Result: The results showed that triploid H. discus hannai had a chromosome number of 3n = 54, consisting of 30 metacentric (m) and 24 submetacentric (sm) chromosomes, while the diploids had a chromosome number of 2n = 36, consisting of 20 metacentric (m) and 16 submetacentric (sm) chromosomes. Notably, both triploids and diploids displayed variation in the number of NORs and/or their diameter. The average number of NORs in triploid was significantly higher than that in diploids (p < 0.05), but the average diameter of NORs of triploid was no significant different from that of diploid (p > 0.05). Additionally, 5S rDNA localization to 3 submetacentric chromosomes was observed in triploids, compared to 2 submetacentric chromosomes in diploids. The number of 18S rDNA sites displayed positional conservancy and quantitative variability in both diploids and triploids. Specifically, 18S rDNA was found at the end of the chromosome in both groups, with triploids exhibiting a significantly higher number of loci than diploids (p < 0.01).

Conclusion: This study provides valuable insights into the cytogenetic characteristics of triploid H. discus hannai, which could facilitate further research on the stability of the chromosome set in this species.

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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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