He Zhao , Xiangbo Liu , Junling Zhang , Wentao Zhu , Chen Su , Aimin Wang , Xiubao Li
{"title":"蛋白质组学和代谢组学的综合分析揭示了主动修复对风信子Acropora hyacinthus的影响","authors":"He Zhao , Xiangbo Liu , Junling Zhang , Wentao Zhu , Chen Su , Aimin Wang , Xiubao Li","doi":"10.1016/j.jembe.2023.151972","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to investigate the impact of active restoration on the physiology and metabolism of <em>Acropora hyacinthus</em> by comparing physiological, proteomic, and metabolomic differences between transplanted and natural corals. The results revealed that transplanted <em>A. hyacinthus</em> exhibited significantly higher quantum yield (F<sub>v</sub>/F<sub>m</sub>) and zooxanthellae density than natural corals. The proteome of the two groups was analyzed qualitatively and quantitatively, and 202 up-regulated and 284 down-regulated differential proteins were found. In addition, the differential metabolites between the two groups were screened and classified, ultimately identifying 314 up-regulated differential metabolites and 29 down-regulated differential metabolites. A comprehensive analysis of the enrichment pathways reveals that three pathways, namely arachidonic acid metabolism, glycerophospholipid metabolism, and cysteine and methionine metabolism, were found to be implicated in both the differential proteins and differential metabolites. Transplanted <em>A. hyacinthus</em> had low levels of (12<em>S</em>)-HETE, which may affect subsequent growth and development of the coral. This study demonstrates that aggressive restoration has contributed to an increase in coral cover, but the health of transplanted corals cannot be overlooked. Proteomics and metabolomics studies can offer essential insights for addressing potential coral health issues.</p></div>","PeriodicalId":50197,"journal":{"name":"Journal of Experimental Marine Biology and Ecology","volume":"570 ","pages":"Article 151972"},"PeriodicalIF":1.8000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022098123001041/pdfft?md5=20aa5f2ffbc5933517786cd6a20947d8&pid=1-s2.0-S0022098123001041-main.pdf","citationCount":"0","resultStr":"{\"title\":\"An integrative analysis of proteomics and metabolomics reveals the effects of active restoration on Acropora hyacinthus\",\"authors\":\"He Zhao , Xiangbo Liu , Junling Zhang , Wentao Zhu , Chen Su , Aimin Wang , Xiubao Li\",\"doi\":\"10.1016/j.jembe.2023.151972\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to investigate the impact of active restoration on the physiology and metabolism of <em>Acropora hyacinthus</em> by comparing physiological, proteomic, and metabolomic differences between transplanted and natural corals. The results revealed that transplanted <em>A. hyacinthus</em> exhibited significantly higher quantum yield (F<sub>v</sub>/F<sub>m</sub>) and zooxanthellae density than natural corals. The proteome of the two groups was analyzed qualitatively and quantitatively, and 202 up-regulated and 284 down-regulated differential proteins were found. In addition, the differential metabolites between the two groups were screened and classified, ultimately identifying 314 up-regulated differential metabolites and 29 down-regulated differential metabolites. A comprehensive analysis of the enrichment pathways reveals that three pathways, namely arachidonic acid metabolism, glycerophospholipid metabolism, and cysteine and methionine metabolism, were found to be implicated in both the differential proteins and differential metabolites. Transplanted <em>A. hyacinthus</em> had low levels of (12<em>S</em>)-HETE, which may affect subsequent growth and development of the coral. This study demonstrates that aggressive restoration has contributed to an increase in coral cover, but the health of transplanted corals cannot be overlooked. Proteomics and metabolomics studies can offer essential insights for addressing potential coral health issues.</p></div>\",\"PeriodicalId\":50197,\"journal\":{\"name\":\"Journal of Experimental Marine Biology and Ecology\",\"volume\":\"570 \",\"pages\":\"Article 151972\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0022098123001041/pdfft?md5=20aa5f2ffbc5933517786cd6a20947d8&pid=1-s2.0-S0022098123001041-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental Marine Biology and Ecology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022098123001041\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Marine Biology and Ecology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022098123001041","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
An integrative analysis of proteomics and metabolomics reveals the effects of active restoration on Acropora hyacinthus
This study aimed to investigate the impact of active restoration on the physiology and metabolism of Acropora hyacinthus by comparing physiological, proteomic, and metabolomic differences between transplanted and natural corals. The results revealed that transplanted A. hyacinthus exhibited significantly higher quantum yield (Fv/Fm) and zooxanthellae density than natural corals. The proteome of the two groups was analyzed qualitatively and quantitatively, and 202 up-regulated and 284 down-regulated differential proteins were found. In addition, the differential metabolites between the two groups were screened and classified, ultimately identifying 314 up-regulated differential metabolites and 29 down-regulated differential metabolites. A comprehensive analysis of the enrichment pathways reveals that three pathways, namely arachidonic acid metabolism, glycerophospholipid metabolism, and cysteine and methionine metabolism, were found to be implicated in both the differential proteins and differential metabolites. Transplanted A. hyacinthus had low levels of (12S)-HETE, which may affect subsequent growth and development of the coral. This study demonstrates that aggressive restoration has contributed to an increase in coral cover, but the health of transplanted corals cannot be overlooked. Proteomics and metabolomics studies can offer essential insights for addressing potential coral health issues.
期刊介绍：
The Journal of Experimental Marine Biology and Ecology provides a forum for experimental ecological research on marine organisms in relation to their environment. Topic areas include studies that focus on biochemistry, physiology, behavior, genetics, and ecological theory. The main emphasis of the Journal lies in hypothesis driven experimental work, both from the laboratory and the field. Natural experiments or descriptive studies that elucidate fundamental ecological processes are welcome. Submissions should have a broad ecological framework beyond the specific study organism or geographic region.
Short communications that highlight emerging issues and exciting discoveries within five printed pages will receive a rapid turnaround. Papers describing important new analytical, computational, experimental and theoretical techniques and methods are encouraged and will be highlighted as Methodological Advances. We welcome proposals for Review Papers synthesizing a specific field within marine ecology. Finally, the journal aims to publish Special Issues at regular intervals synthesizing a particular field of marine science. All printed papers undergo a peer review process before being accepted and will receive a first decision within three months.
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