Shawn Miller Jr , Edgar Juarez Lopez , Jessica M.L. Grittner , Brendan J. Dougherty
{"title":"低水平的二氧化碳补充维持异氧血症,揭示大鼠的通气长期促进作用","authors":"Shawn Miller Jr , Edgar Juarez Lopez , Jessica M.L. Grittner , Brendan J. Dougherty","doi":"10.1016/j.resp.2023.104185","DOIUrl":null,"url":null,"abstract":"<div><p>Acute, intermittent hypoxia (AIH) induces ventilatory long-term facilitation (vLTF) in awake, freely behaving rats under poikilocapnic and isocapnic experimental conditions. Establishing pre-clinical methods for vLTF induction that more closely align with successful protocols in humans and anesthetized rats would minimize dissonance in experimental findings and improve translational aspects of vLTF. Here, we tested several levels of low-dose CO<sub>2</sub> supplementation during and after AIH to determine 1) the lowest amount of inspired CO<sub>2</sub> that would maintain isocapnia in rats during a vLTF protocol, and 2) the net impact of supplemental CO<sub>2</sub> on vLTF expression. Rats received one of four levels of inspired CO<sub>2</sub> (0%, 0.5%, 1% or 2%) administered during AIH and for the 60 min following AIH to quantify vLTF. Our findings indicated that 2% inspired CO<sub>2</sub> was sufficient to maintain isocapnia across the AIH protocol and reveal significant vLTF. These findings provide evidence-based support for using 2% supplemental CO<sub>2</sub> during and after AIH when assessing vLTF in rats.</p></div>","PeriodicalId":20961,"journal":{"name":"Respiratory Physiology & Neurobiology","volume":"320 ","pages":"Article 104185"},"PeriodicalIF":1.9000,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1569904823001738/pdfft?md5=0f56e4a33e227a009bb3e20619e4e658&pid=1-s2.0-S1569904823001738-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Low level CO2 supplementation maintains isocapnia and reveals ventilatory long-term facilitation in rats\",\"authors\":\"Shawn Miller Jr , Edgar Juarez Lopez , Jessica M.L. Grittner , Brendan J. Dougherty\",\"doi\":\"10.1016/j.resp.2023.104185\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Acute, intermittent hypoxia (AIH) induces ventilatory long-term facilitation (vLTF) in awake, freely behaving rats under poikilocapnic and isocapnic experimental conditions. Establishing pre-clinical methods for vLTF induction that more closely align with successful protocols in humans and anesthetized rats would minimize dissonance in experimental findings and improve translational aspects of vLTF. Here, we tested several levels of low-dose CO<sub>2</sub> supplementation during and after AIH to determine 1) the lowest amount of inspired CO<sub>2</sub> that would maintain isocapnia in rats during a vLTF protocol, and 2) the net impact of supplemental CO<sub>2</sub> on vLTF expression. Rats received one of four levels of inspired CO<sub>2</sub> (0%, 0.5%, 1% or 2%) administered during AIH and for the 60 min following AIH to quantify vLTF. Our findings indicated that 2% inspired CO<sub>2</sub> was sufficient to maintain isocapnia across the AIH protocol and reveal significant vLTF. These findings provide evidence-based support for using 2% supplemental CO<sub>2</sub> during and after AIH when assessing vLTF in rats.</p></div>\",\"PeriodicalId\":20961,\"journal\":{\"name\":\"Respiratory Physiology & Neurobiology\",\"volume\":\"320 \",\"pages\":\"Article 104185\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-11-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1569904823001738/pdfft?md5=0f56e4a33e227a009bb3e20619e4e658&pid=1-s2.0-S1569904823001738-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Respiratory Physiology & Neurobiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1569904823001738\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Respiratory Physiology & Neurobiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1569904823001738","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
Low level CO2 supplementation maintains isocapnia and reveals ventilatory long-term facilitation in rats
Acute, intermittent hypoxia (AIH) induces ventilatory long-term facilitation (vLTF) in awake, freely behaving rats under poikilocapnic and isocapnic experimental conditions. Establishing pre-clinical methods for vLTF induction that more closely align with successful protocols in humans and anesthetized rats would minimize dissonance in experimental findings and improve translational aspects of vLTF. Here, we tested several levels of low-dose CO2 supplementation during and after AIH to determine 1) the lowest amount of inspired CO2 that would maintain isocapnia in rats during a vLTF protocol, and 2) the net impact of supplemental CO2 on vLTF expression. Rats received one of four levels of inspired CO2 (0%, 0.5%, 1% or 2%) administered during AIH and for the 60 min following AIH to quantify vLTF. Our findings indicated that 2% inspired CO2 was sufficient to maintain isocapnia across the AIH protocol and reveal significant vLTF. These findings provide evidence-based support for using 2% supplemental CO2 during and after AIH when assessing vLTF in rats.
期刊介绍:
Respiratory Physiology & Neurobiology (RESPNB) publishes original articles and invited reviews concerning physiology and pathophysiology of respiration in its broadest sense.
Although a special focus is on topics in neurobiology, high quality papers in respiratory molecular and cellular biology are also welcome, as are high-quality papers in traditional areas, such as:
-Mechanics of breathing-
Gas exchange and acid-base balance-
Respiration at rest and exercise-
Respiration in unusual conditions, like high or low pressure or changes of temperature, low ambient oxygen-
Embryonic and adult respiration-
Comparative respiratory physiology.
Papers on clinical aspects, original methods, as well as theoretical papers are also considered as long as they foster the understanding of respiratory physiology and pathophysiology.