Sandeep Kumar, Nalla Sai Praneet, Kitlangki Suchiang
{"title":"短乳杆菌MTCC 1750通过DAF-16途径增强秀丽隐杆线虫的氧化应激抗性和延长寿命,改善其生理和功能能力。","authors":"Sandeep Kumar, Nalla Sai Praneet, Kitlangki Suchiang","doi":"10.1080/10715762.2022.2155518","DOIUrl":null,"url":null,"abstract":"<p><p>Redox imbalance plays a crucial role in the development of age-related diseases, and resistance to oxidative stress is crucial for optimum longevity and healthy aging. Using the wild-type, mutant and transgenic strains, this study explored the antioxidative potential and lifespan extension benefits of different <i>Lactobacillus strains</i> in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). We observed that <i>Lactobacillus brevis</i> MTCC 1750 could enhance the resistance of <i>C. elegans</i> against juglone induced oxidative stress by reducing its intracellular reactive oxygen species (ROS) accumulation. Also, live <i>L. brevis</i> MTCC 1750 could prolong the worm's lifespan. These effects are dependent on transcription factor DAF-16 evident with significant upregulation of its target gene <i>sod-3</i>. This also explained the significant improvements in different age-associated changes in physiological and mechanical parameters of the worm by <i>L. brevis</i> MTCC 1750. Further investigations revealed that DAF-16 activation and, its enhanced translocation in the nucleus is independent of DAF-2 or JNK pathway. These findings highlighted <i>L. brevis</i> MTCC 1750 as a potent anti-oxidant source for complementing current antioxidant therapeutic strategies. Nonetheless, the findings showed how different signaling events are regulated based on an organism's diet component, and their consequences on the aging process in multiple species.</p>","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"<i>Lactobacillus brevis</i> MTCC 1750 enhances oxidative stress resistance and lifespan extension with improved physiological and functional capacity in <i>Caenorhabditis elegans</i> via the DAF-16 pathway.\",\"authors\":\"Sandeep Kumar, Nalla Sai Praneet, Kitlangki Suchiang\",\"doi\":\"10.1080/10715762.2022.2155518\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Redox imbalance plays a crucial role in the development of age-related diseases, and resistance to oxidative stress is crucial for optimum longevity and healthy aging. Using the wild-type, mutant and transgenic strains, this study explored the antioxidative potential and lifespan extension benefits of different <i>Lactobacillus strains</i> in <i>Caenorhabditis elegans</i> (<i>C. elegans</i>). We observed that <i>Lactobacillus brevis</i> MTCC 1750 could enhance the resistance of <i>C. elegans</i> against juglone induced oxidative stress by reducing its intracellular reactive oxygen species (ROS) accumulation. Also, live <i>L. brevis</i> MTCC 1750 could prolong the worm's lifespan. These effects are dependent on transcription factor DAF-16 evident with significant upregulation of its target gene <i>sod-3</i>. This also explained the significant improvements in different age-associated changes in physiological and mechanical parameters of the worm by <i>L. brevis</i> MTCC 1750. Further investigations revealed that DAF-16 activation and, its enhanced translocation in the nucleus is independent of DAF-2 or JNK pathway. These findings highlighted <i>L. brevis</i> MTCC 1750 as a potent anti-oxidant source for complementing current antioxidant therapeutic strategies. Nonetheless, the findings showed how different signaling events are regulated based on an organism's diet component, and their consequences on the aging process in multiple species.</p>\",\"PeriodicalId\":12411,\"journal\":{\"name\":\"Free Radical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2022-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Free Radical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/10715762.2022.2155518\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Free Radical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/10715762.2022.2155518","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Lactobacillus brevis MTCC 1750 enhances oxidative stress resistance and lifespan extension with improved physiological and functional capacity in Caenorhabditis elegans via the DAF-16 pathway.
Redox imbalance plays a crucial role in the development of age-related diseases, and resistance to oxidative stress is crucial for optimum longevity and healthy aging. Using the wild-type, mutant and transgenic strains, this study explored the antioxidative potential and lifespan extension benefits of different Lactobacillus strains in Caenorhabditis elegans (C. elegans). We observed that Lactobacillus brevis MTCC 1750 could enhance the resistance of C. elegans against juglone induced oxidative stress by reducing its intracellular reactive oxygen species (ROS) accumulation. Also, live L. brevis MTCC 1750 could prolong the worm's lifespan. These effects are dependent on transcription factor DAF-16 evident with significant upregulation of its target gene sod-3. This also explained the significant improvements in different age-associated changes in physiological and mechanical parameters of the worm by L. brevis MTCC 1750. Further investigations revealed that DAF-16 activation and, its enhanced translocation in the nucleus is independent of DAF-2 or JNK pathway. These findings highlighted L. brevis MTCC 1750 as a potent anti-oxidant source for complementing current antioxidant therapeutic strategies. Nonetheless, the findings showed how different signaling events are regulated based on an organism's diet component, and their consequences on the aging process in multiple species.
期刊介绍:
Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.