{"title":"肝炎抗凝乳法","authors":"W. Lange , K.N. Masihi","doi":"10.1016/S0172-5599(80)80033-2","DOIUrl":null,"url":null,"abstract":"<div><p>An enzyme immunoassay (ELISA) from SEROMED was tested for ist suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis.</p><p>From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higher than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somewhat lower sensitivity of ELISA.</p><p>However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA.</p><p>ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 3","pages":"Pages 296-302"},"PeriodicalIF":0.0000,"publicationDate":"1980-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80033-2","citationCount":"0","resultStr":"{\"title\":\"Der Nachweis von Hepatitis Be-Antigen mit einem Enzymimmunoassay\",\"authors\":\"W. Lange , K.N. Masihi\",\"doi\":\"10.1016/S0172-5599(80)80033-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An enzyme immunoassay (ELISA) from SEROMED was tested for ist suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis.</p><p>From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higher than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somewhat lower sensitivity of ELISA.</p><p>However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA.</p><p>ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.</p></div>\",\"PeriodicalId\":101293,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"volume\":\"247 3\",\"pages\":\"Pages 296-302\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80033-2\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0172559980800332\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0172559980800332","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Der Nachweis von Hepatitis Be-Antigen mit einem Enzymimmunoassay
An enzyme immunoassay (ELISA) from SEROMED was tested for ist suitability in detecting HBeAg and was compared with a radioimmunoassay (RIA) from ABBOTT and with rheophoresis.
From a total of 931 sera tested, HBeAg could be detected in 313 sera with ELISA, 347 sera with RIA and 22 sera with rheophoresis. When 8 HBeAg containing sera were titrated for end points, RIA gave titers which were 2.3-fold higher than ELISA and 575-fold higher than rheophoresis. The differences between RIA and ELISA were shown to be due to somewhat lower sensitivity of ELISA.
However, when sera from patients with Hepatitis B from various times after the onset of hepatitis (maximally 12 months) were tested, there was only a slight difference in sensitivity between ELISA and RIA.
ELISA along with RIA is considered to be a substantially sensitive test for detection of HBeAG as compared to rheophoresis and can be recommended for routine use.
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