薄层色谱快速检测拟杆菌对碳水化合物的利用

Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1980-08-01 DOI:10.1016/S0172-5599(80)80049-6

Rainer Hammann , Ingrid Werner

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引用次数: 4

摘要

采用薄层色谱法(TLC)测定了19株不同种类的弱溶糖拟杆菌菌株对果糖、蔗糖、乳糖、半乳糖和棉子糖的发酵能力。传统的发酵试验是并行进行的。总的来说，两种方法之间的一致性很好。然而，两种菌株在TLC测试中显示出没有酸化的降解。在一些菌株中，蔗糖作为底物产生果糖点，乳糖产生半乳糖点，棉子糖产生糖二糖点，表明这些碳水化合物的降解不完全。

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Thin Layer Chromatography for Rapid Detection of Carbohydrate Utilization by Bacteroides Strains

19 weakly saccharolytic Bacteroides strains of different species were tested by thin-layer

chromatography (TLC) for their fermentative abilities for fructose, sucrose, lactose, galactose, and raffinose. Conventional fermentation tests were run in parallel. In general, a good agreement between both methods was recorded. Two strains, however, showed a degradation in the TLC test without an acidification. With some strains, sucrose as a substrate yielded a fructose spot, lactose a galactose spot, and raffinose a melibiose spot, indicating an incomplete degradation of these carbohydrates.

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Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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