[使用与数字颗粒成像装置相结合的自动尿液颗粒分析仪检测和识别尿晶体]。

IF 0.4 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Carole Hennequin, Laurie Candelot, Agnès Ferroni, Sandrine Majoux, Jean-Louis Beaudeux, Valérie Nivet-Antoine
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引用次数: 0

摘要

偏振光显微镜(POM)仍然是分析结晶尿的金标准。然而,这种方法是耗时的,需要训练有素的工作人员。在这里,为了解决这个问题,我们测试了连接到UD10模块的Sysmex UF-4000分析仪作为自动流式细胞术-数字颗粒成像工作流程,以评估(i)系统检测和识别晶体种类的能力以及(ii)由UD-10模块提供的图像质量(n = 40)为分析的每个尿液样本。首先,用POM和UF-4000/UD-10分析仪对76份样品进行系统分析,结果表明,只需仔细检查40张照片,就可以自信地检测出结晶尿阳性样品,没有遗漏,从而可以区分阳性结晶尿和阴性结晶尿。这些最初的结果通过敏感性分析和对200个样品计算的阴性预测值(由UF-4000提供的结果分别为39%和46%)以及对40张UD-10照片的检查(两个值均为100%)得到证实。因此,数字图像可以用于筛查结晶尿而不会丢失晶体。部分样本在不必要的情况下进行了POM处理(阳性预测值:78%)。最后,我们比较了Sysmex UF4000/UD10工作流程和“金标准”POM方法对131份含晶体尿液样本的晶体鉴定性能。Sysmex UF-4000只鉴定出草酸钙二水合物晶体。对数码照片的仔细检查使84.7%的样品能够准确地识别出晶体,这表明只要在照片上观察到晶体,就仍然需要POM。我们得出结论,SYSMEX UF-4000结合UD-10模块可以在实践中使用,通过仔细检查照片来区分阳性结晶尿和阴性结晶尿。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Detection and identification of urinary crystals using an automated urine particle analyzer coupled with a digital particle imaging device].

Polarized light microscopy (POM) remains the gold standard for crystalluria analysis. However, such method is time consuming and requires well-trained staff. Here, to address this issue, we tested the Sysmex UF-4000 analyzer coupled to a UD10 module as an automated flow cytometry-digital particle imaging workflow to assess (i) the ability of the system to detect and identify the crystals species and (ii) the quality of the images provided by the UD-10 module (n = 40) for each urine sample analyzed. First, systematic analysis of 76 samples by POM and the UF-4000/UD-10 analyzer showed that only attentive examination of the 40 photos was able to confidently detect crystalluria-positive samples with no misses and thus serve to discriminate positive-test crystalluria from negative-test crystalluria. These first results were confirmed by sensitivity analysis and the negative predictive value calculated on 200 samples for the results provided by the UF-4000 (39% and 46%) and after examination of the 40 UD-10 photos (100% for the both values). Digital images can therefore serve to screen crystalluria without missing crystals. A part of samples were treated by POM whereas it was not necessary (positive predictive value: 78%). Finally, we compared the crystal identification performances of the Sysmex UF4000/UD10 workflow and the ‘gold standard’ POM method on 131 urine samples containing crystals. Only calcium oxalate dihydrate crystals were identified by the Sysmex UF-4000. A close examination of the digital photographs enabled exact identification of crystals in 84.7% of the samples, suggesting however that POM is still require as soon as crystals are observed on the photographs. We conclude that a SYSMEX UF-4000 coupled with a UD-10 module can be used in practice with close examination of the photographs to discriminate positive crystalluria from negative crystalluria.

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来源期刊
Annales de biologie clinique
Annales de biologie clinique 医学-医学:研究与实验
CiteScore
0.80
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Multidisciplinary information with direct relevance to everyday practice Annales de Biologie Clinique, the official journal of the French Society of Clinical Biology (SFBC), supports biologists in areas including continuing education, laboratory accreditation and technique validation. With original articles, abstracts and accounts of everyday practice, the journal provides details of advances in knowledge, techniques and equipment, as well as a forum for discussion open to the entire community.
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