使用质粒DNA的寡核苷酸定向诱变:确认正向诱变成功的第一步的筛选步骤

Technical Tips Online Pub Date : 2001-01-01 DOI:10.1016/S1366-2120(08)70159-4

Anthony M. Smith

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引用次数: 0

摘要

定点突变(site-directed mutagenesis, SDM)技术在分子生物学中被广泛应用于将突变引入DNA (Ref.[1])。本文讨论了一种可以纳入诱变系统的快速筛选步骤，例如{GeneEditor体外定点诱变系统(Promega, Madison, WI, USA)}，该系统操纵克隆到大肠杆菌质粒中的目标DNA，并使用抗生素选择来选择突变体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Oligonucleotide-directed mutagenesis using plasmid DNA: a screening step to confirm the first successful step to positive mutagenesis

The technique of site-directed mutagenesis (SDM) is widely used in molecular biology to introduce mutations into DNA (Ref. [1]). This article discusses a quick screening step that can be incorporated into mutagenesis systems such as the {GeneEditor in vitro site-directed mutagenesis system (Promega, Madison, WI, USA)}, which manipulates target DNA cloned into an Escherichia coli plasmid and uses antibiotic selection to select for mutants.

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Technical Tips Online

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