聚赖氨酸与人红细胞膜可溶性组分的相互作用

D. Danon , C. Howe, L.T. Lee
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引用次数: 32

摘要

1.1. 用琼脂凝胶电泳和免疫电泳研究了聚赖氨酸与可溶性红细胞组分和禽源性唾液蛋白Collocalia mucoid的相互作用。在琼脂凝胶中,聚赖氨酸与红细胞膜糖蛋白和结肠黏液结合形成可见的沉淀物;缺乏糖蛋白的红细胞部分也没有反应。通过在混合物中加入聚l-天冬氨酸,可以定量地再溶解含有聚赖氨酸的病毒受体物质沉淀物。这种溶解度的变化是由于糖蛋白中聚赖氨酸的取代还是由于可溶性复合物的形成而发生的,目前还不能确定。神经氨酸酶(EC 3.2.1.18)对红细胞糖蛋白和结肠黏液的处理大大降低或消除了它们与聚赖氨酸沉淀的能力。研究结果表明,红细胞膜酸性糖蛋白中的n -乙酰神经氨酸是红细胞被多碱基凝集的重要因素,这种现象被认为与血管内血栓形成有一定关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Interaction of polylysine with soluble components of human erythrocyte membranes

  • 1.

    1. The interactions of poly-l-lysine with soluble erythrocyte components and with Collocalia mucoid, a sialoprotein of avian origin, were studied by electrophoresis and immuno-electrophoresis in agar gel.

  • 2.

    2. Polylysine combined with erythrocyte membrane glycoprotein and with Collocalia mucoid to form visible precipitates in agar gel; erythrocyte fractions devoid of glycoprotein failed so to react.

  • 3.

    3. A precipitate of virus receptor substance with polylysine could be quantitatively redissolved by the addition of poly-l-aspartic acid to the mixture. Whether this change in solubility occurred as a result of displacement of polylysine from the glycoprotein or by the formation of soluble complexes could not be established.

  • 4.

    4. Neuraminidase (EC 3.2.1.18) treatment of erythrocyte glycoproteins and Collocalia mucoid greatly reduced or abolished their capacity to precipitate with polylysine.

  • 5.

    5. The findings suggest that N-acetylneuraminic acid in the acid glycoprotein of erythrocyte membranes is an important factor in the agglutination of erythrocytes by polybases, a phenomenon thought to have some bearing on intravascular thrombus formation.

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