基尔库克市餐馆工人鼻中分离的耐甲氧西林金黄色葡萄球菌中Panton-Valentine白细胞调素和中毒性休克综合征毒素-1基因的患病率。

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics
Najdat Bahjat Mahdi
{"title":"基尔库克市餐馆工人鼻中分离的耐甲氧西林金黄色葡萄球菌中Panton-Valentine白细胞调素和中毒性休克综合征毒素-1基因的患病率。","authors":"Najdat Bahjat Mahdi","doi":"10.4103/japtr.japtr_508_22","DOIUrl":null,"url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> resides naturally in the nasal cavity of healthy individuals, including those working in restaurants, so they may be a source for spreading this bacterium to restaurant customers directly or indirectly through cooked meals. This bacterium has several virulence factors enabling it to cause many diseases in different parts of the body. It has also the capability to resist conventional antibiotics including methicillin. To investigate methicillin-resistant <i>S. aureus</i> (MRSA), 170 nasal swabs were collected from food preparation workers in 30 restaurants (5-6 workers in each restaurant) in Kirkuk city. After collection, the samples were directly transferred to the laboratory and cultured on selective media like mannitol salt agar (MSA). Microbiological examination including morphological, biochemical, and confirmatory tests showed that 24/170 of collected samples were positive for <i>S. aureus</i> with a rate of 14.12%. Among 24 isolates, 20 (83.3%) belonged to MRSA. All isolates were resistant to oxacillin and penicillin (100%), whereas sensitive to other antibiotics (gentamicin, chloramphenicol, and rifampicin). Polymerase chain reaction exhibited that 13 (65%) of MRSA isolates have toxic shock syndrome toxin-1 gene and only 4 (20%) have Panton-Valentine leukocidin gene.</p>","PeriodicalId":14877,"journal":{"name":"Journal of Advanced Pharmaceutical Technology & Research","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/59/JAPTR-14-34.PMC10026320.pdf","citationCount":"0","resultStr":"{\"title\":\"Prevalence of Panton-Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant <i>Staphylococcus aureus</i> isolated from nose of restaurant workers in Kirkuk city.\",\"authors\":\"Najdat Bahjat Mahdi\",\"doi\":\"10.4103/japtr.japtr_508_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Staphylococcus aureus</i> resides naturally in the nasal cavity of healthy individuals, including those working in restaurants, so they may be a source for spreading this bacterium to restaurant customers directly or indirectly through cooked meals. This bacterium has several virulence factors enabling it to cause many diseases in different parts of the body. It has also the capability to resist conventional antibiotics including methicillin. To investigate methicillin-resistant <i>S. aureus</i> (MRSA), 170 nasal swabs were collected from food preparation workers in 30 restaurants (5-6 workers in each restaurant) in Kirkuk city. After collection, the samples were directly transferred to the laboratory and cultured on selective media like mannitol salt agar (MSA). Microbiological examination including morphological, biochemical, and confirmatory tests showed that 24/170 of collected samples were positive for <i>S. aureus</i> with a rate of 14.12%. Among 24 isolates, 20 (83.3%) belonged to MRSA. All isolates were resistant to oxacillin and penicillin (100%), whereas sensitive to other antibiotics (gentamicin, chloramphenicol, and rifampicin). Polymerase chain reaction exhibited that 13 (65%) of MRSA isolates have toxic shock syndrome toxin-1 gene and only 4 (20%) have Panton-Valentine leukocidin gene.</p>\",\"PeriodicalId\":14877,\"journal\":{\"name\":\"Journal of Advanced Pharmaceutical Technology & Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/59/JAPTR-14-34.PMC10026320.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Advanced Pharmaceutical Technology & Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/japtr.japtr_508_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Pharmaceutical Technology & Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/japtr.japtr_508_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0

摘要

金黄色葡萄球菌自然存在于健康人的鼻腔中,包括那些在餐馆工作的人,因此它们可能是通过熟食直接或间接将这种细菌传播给餐馆顾客的来源。这种细菌有几种毒力因子,使其能够在身体的不同部位引起许多疾病。它还具有抵抗包括甲氧西林在内的常规抗生素的能力。为了调查耐甲氧西林金黄色葡萄球菌(MRSA),从基尔库克市30家餐馆的食品制备工人(每家餐馆5-6名工人)身上采集了170份鼻拭子。采集后,将样品直接转移到实验室,并在甘露醇盐琼脂(MSA)等选择性培养基上培养。包括形态、生化和确证测试在内的微生物检查显示,采集的样品中有24/170对金黄色葡萄球菌呈阳性,阳性率为14.12%。在24个分离株中,20个(83.3%)属于MRSA。所有分离株均对苯唑西林和青霉素具有耐药性(100%),而对其他抗生素(庆大霉素、氯霉素和利福平)敏感。聚合酶链反应显示,MRSA分离株中有13株(65%)具有中毒性休克综合征毒素-1基因,只有4株(20%)具有Panton Valentine白细胞调素基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Prevalence of Panton-Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant <i>Staphylococcus aureus</i> isolated from nose of restaurant workers in Kirkuk city.

Prevalence of Panton-Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant <i>Staphylococcus aureus</i> isolated from nose of restaurant workers in Kirkuk city.

Prevalence of Panton-Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant <i>Staphylococcus aureus</i> isolated from nose of restaurant workers in Kirkuk city.

Prevalence of Panton-Valentine leukocidin and toxic shock syndrome toxin-1 genes in methicillin-resistant Staphylococcus aureus isolated from nose of restaurant workers in Kirkuk city.

Staphylococcus aureus resides naturally in the nasal cavity of healthy individuals, including those working in restaurants, so they may be a source for spreading this bacterium to restaurant customers directly or indirectly through cooked meals. This bacterium has several virulence factors enabling it to cause many diseases in different parts of the body. It has also the capability to resist conventional antibiotics including methicillin. To investigate methicillin-resistant S. aureus (MRSA), 170 nasal swabs were collected from food preparation workers in 30 restaurants (5-6 workers in each restaurant) in Kirkuk city. After collection, the samples were directly transferred to the laboratory and cultured on selective media like mannitol salt agar (MSA). Microbiological examination including morphological, biochemical, and confirmatory tests showed that 24/170 of collected samples were positive for S. aureus with a rate of 14.12%. Among 24 isolates, 20 (83.3%) belonged to MRSA. All isolates were resistant to oxacillin and penicillin (100%), whereas sensitive to other antibiotics (gentamicin, chloramphenicol, and rifampicin). Polymerase chain reaction exhibited that 13 (65%) of MRSA isolates have toxic shock syndrome toxin-1 gene and only 4 (20%) have Panton-Valentine leukocidin gene.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信