贵金属沉积TiO2纳米棒暴露对哺乳动物精子失活的影响。

IF 2.5 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Young-Joo Yi, Love Kumar Dhandole, Dong-Won Seo, Sang-Myeong Lee, Jum Suk Jang
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引用次数: 0

摘要

二氧化钛(TiO2)纳米棒是一种广泛应用的半导体和催化材料,但其毒性也引起了人们的关注。在本研究中,我们研究并比较了TiO2 NRs和负载Ag或Au NPs的TiO2 NRs对猪精子的毒性作用。结果表明,与对照组(含或不含TiO2 NRs)或Au-TiO2 NRs孵育的精子相比,Ag-TiO2 NRs孵育的精子活力较低。Ag-TiO2 NRs存在时,精子活力和顶体完整性受损,且在20 μg/ml Ag-TiO2 NRs的作用下,精子胞内活性氧(ROS)的生成显著增加。我们深入讨论了在精子中ROS生成的背景下,酶促NADPH和Ag-TiO2 NRs之间的电荷转移机制。我们观察到的影响反映了Ag-TiO2 NRs孵育精子的受精能力;Ag-TiO2 NRs降低了体外受精的精子穿透率和胚胎发育率。总之,我们的研究结果表明,暴露于Ag-TiO2 NRs可能会影响雄性的受精能力,并且在使用这些NRs时要谨慎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Inactivation of mammalian spermatozoa on the exposure of TiO<sub>2</sub> nanorods deposited with noble metals.

Inactivation of mammalian spermatozoa on the exposure of TiO<sub>2</sub> nanorods deposited with noble metals.

Inactivation of mammalian spermatozoa on the exposure of TiO<sub>2</sub> nanorods deposited with noble metals.

Inactivation of mammalian spermatozoa on the exposure of TiO2 nanorods deposited with noble metals.

Titanium dioxide (TiO2) nanorods (NRs) are well-known semiconducting and catalytic material that has been widely applied, but their toxicities have also attracted recent interest. In this study, we investigated and compared the toxic effects of TiO2 NRs and TiO2 NRs loaded with Ag or Au NPs on boar spermatozoa. As a result, sperm incubated with Ag-TiO2 NRs showed lower motility than sperm incubated with controls (with or without TiO2 NRs) or Au-TiO2 NRs. In addition, sperm viability and acrosomal integrity were defective in the presence of Ag-TiO2 NRs, and the generation of intracellular reactive oxygen species (ROS) increased significantly when spermatozoa were incubated with 20 μg/ml Ag-TiO2 NRs. We discussed in depth the charge transfer mechanism between enzymatic NADPH and Ag-TiO2 NRs in the context of ROS generation in spermatozoa. The effects we observed reflected the fertilization competence of sperm incubated with Ag-TiO2 NRs; specifically sperm penetration and embryonic development rates by in vitro fertilization were reduced by Ag-TiO2 NRs. To summarize, our findings indicate that exposure to Ag-TiO2 NRs could affect male fertilization fecundity and caution that care be exercised when using these NRs.

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来源期刊
Journal of Analytical Science and Technology
Journal of Analytical Science and Technology Environmental Science-General Environmental Science
CiteScore
4.00
自引率
4.20%
发文量
39
审稿时长
13 weeks
期刊介绍: The Journal of Analytical Science and Technology (JAST) is a fully open access peer-reviewed scientific journal published under the brand SpringerOpen. JAST was launched by Korea Basic Science Institute in 2010. JAST publishes original research and review articles on all aspects of analytical principles, techniques, methods, procedures, and equipment. JAST’s vision is to be an internationally influential and widely read analytical science journal. Our mission is to inform and stimulate researchers to make significant professional achievements in science. We aim to provide scientists, researchers, and students worldwide with unlimited access to the latest advances of the analytical sciences.
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