蜘蛛Phonotimpus pennimani转录组分析揭示了新的毒素转录物。

IF 1.8 3区 医学 Q4 TOXICOLOGY
Jonathan David Baza-Moreno, Leticia Vega-Alvarado, Guillermo Ibarra-Núñez, Karina Guillén-Navarro, Luz Verónica García-Fajardo, Verónica Jiménez-Jacinto, Elia Diego-García
{"title":"蜘蛛Phonotimpus pennimani转录组分析揭示了新的毒素转录物。","authors":"Jonathan David Baza-Moreno,&nbsp;Leticia Vega-Alvarado,&nbsp;Guillermo Ibarra-Núñez,&nbsp;Karina Guillén-Navarro,&nbsp;Luz Verónica García-Fajardo,&nbsp;Verónica Jiménez-Jacinto,&nbsp;Elia Diego-García","doi":"10.1590/1678-9199-JVATITD-2022-0031","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Phonotimpus pennimani</i> (Araneae, Phrurolithidae) is a small-sized (3-5 mm) spider endemic to the Tacaná volcano in Chiapas, Mexico, where it is found in soil litter of cloud forests and coffee plantations. Its venom composition has so far not been investigated, partly because it is not a species of medical significance. However, it does have an important impact on the arthropod populations of its natural habitat.</p><p><strong>Methods: </strong>Specimens were collected in Southeastern Mexico (Chiapas) and identified taxonomically by morphological characteristics. A partial sequence from the mitochondrial gene <i>coxI</i> was amplified. Sequencing on the Illumina platform of a transcriptome library constructed from 12 adult specimens revealed 25 toxin or toxin-like genes. Transcripts were validated (RT-qPCR) by assessing the differential expression of the toxin-like PpenTox1 transcript and normalising with housekeeping genes.</p><p><strong>Results: </strong>Analysis of the <i>coxI</i>-gene revealed a similarity to other species of the family Phrurolithidae. Transcriptome analysis also revealed similarity with venom components of species from the families Ctenidae, Lycosidae, and Sicariidae. Expression of the toxin-like PpenTox1 gene was different for each developmental stage (juvenile or adult) and also for both sexes (female or male). Additionally, a partial sequence was obtained for the toxin-like PpenTox1 from DNA.</p><p><strong>Conclusion: </strong>Data from the amplification of the mitochondrial <i>coxI</i> gene confirmed that <i>P. pennimani</i> belongs to the family Phrurolithidae. New genes and transcripts coding for venom components were identified.</p>","PeriodicalId":17565,"journal":{"name":"Journal of Venomous Animals and Toxins Including Tropical Diseases","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881743/pdf/","citationCount":"1","resultStr":"{\"title\":\"Transcriptome analysis of the spider <i>Phonotimpus pennimani</i> reveals novel toxin transcripts.\",\"authors\":\"Jonathan David Baza-Moreno,&nbsp;Leticia Vega-Alvarado,&nbsp;Guillermo Ibarra-Núñez,&nbsp;Karina Guillén-Navarro,&nbsp;Luz Verónica García-Fajardo,&nbsp;Verónica Jiménez-Jacinto,&nbsp;Elia Diego-García\",\"doi\":\"10.1590/1678-9199-JVATITD-2022-0031\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Phonotimpus pennimani</i> (Araneae, Phrurolithidae) is a small-sized (3-5 mm) spider endemic to the Tacaná volcano in Chiapas, Mexico, where it is found in soil litter of cloud forests and coffee plantations. Its venom composition has so far not been investigated, partly because it is not a species of medical significance. However, it does have an important impact on the arthropod populations of its natural habitat.</p><p><strong>Methods: </strong>Specimens were collected in Southeastern Mexico (Chiapas) and identified taxonomically by morphological characteristics. A partial sequence from the mitochondrial gene <i>coxI</i> was amplified. Sequencing on the Illumina platform of a transcriptome library constructed from 12 adult specimens revealed 25 toxin or toxin-like genes. Transcripts were validated (RT-qPCR) by assessing the differential expression of the toxin-like PpenTox1 transcript and normalising with housekeeping genes.</p><p><strong>Results: </strong>Analysis of the <i>coxI</i>-gene revealed a similarity to other species of the family Phrurolithidae. Transcriptome analysis also revealed similarity with venom components of species from the families Ctenidae, Lycosidae, and Sicariidae. Expression of the toxin-like PpenTox1 gene was different for each developmental stage (juvenile or adult) and also for both sexes (female or male). Additionally, a partial sequence was obtained for the toxin-like PpenTox1 from DNA.</p><p><strong>Conclusion: </strong>Data from the amplification of the mitochondrial <i>coxI</i> gene confirmed that <i>P. pennimani</i> belongs to the family Phrurolithidae. New genes and transcripts coding for venom components were identified.</p>\",\"PeriodicalId\":17565,\"journal\":{\"name\":\"Journal of Venomous Animals and Toxins Including Tropical Diseases\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9881743/pdf/\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Venomous Animals and Toxins Including Tropical Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1590/1678-9199-JVATITD-2022-0031\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Venomous Animals and Toxins Including Tropical Diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1590/1678-9199-JVATITD-2022-0031","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 1

摘要

背景:Phonotimpus pennimani(蜘蛛目,栉蛛科)是墨西哥恰帕斯州tacan火山特有的一种小型(3-5毫米)蜘蛛,在云雾林和咖啡种植园的土壤垃圾中发现。到目前为止,它的毒液成分还没有被研究过,部分原因是它不是一个具有医学意义的物种。然而,它确实对其自然栖息地的节肢动物种群有重要影响。方法:在墨西哥东南部(恰帕斯州)采集标本,根据形态特征进行分类鉴定。扩增了线粒体基因coxI的部分序列。在Illumina平台上对12个成年标本构建的转录组文库进行测序,发现25个毒素或毒素样基因。通过评估毒素样PpenTox1转录物的差异表达并与管家基因进行正常化,转录物被验证(RT-qPCR)。结果:对coxi基因的分析显示其与科其他种具有相似性。转录组分析还揭示了蛇麻科、蛇麻科和刺麻科物种毒液成分的相似性。毒素样PpenTox1基因在不同发育阶段(幼年或成年)以及两性(雌性或雄性)的表达不同。此外,从DNA中获得了毒素样PpenTox1的部分序列。结论:线粒体coxI基因扩增结果证实,penimani属栉水母科(Phrurolithidae)。发现了新的毒液成分编码基因和转录本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Transcriptome analysis of the spider <i>Phonotimpus pennimani</i> reveals novel toxin transcripts.

Transcriptome analysis of the spider <i>Phonotimpus pennimani</i> reveals novel toxin transcripts.

Transcriptome analysis of the spider <i>Phonotimpus pennimani</i> reveals novel toxin transcripts.

Transcriptome analysis of the spider Phonotimpus pennimani reveals novel toxin transcripts.

Background: Phonotimpus pennimani (Araneae, Phrurolithidae) is a small-sized (3-5 mm) spider endemic to the Tacaná volcano in Chiapas, Mexico, where it is found in soil litter of cloud forests and coffee plantations. Its venom composition has so far not been investigated, partly because it is not a species of medical significance. However, it does have an important impact on the arthropod populations of its natural habitat.

Methods: Specimens were collected in Southeastern Mexico (Chiapas) and identified taxonomically by morphological characteristics. A partial sequence from the mitochondrial gene coxI was amplified. Sequencing on the Illumina platform of a transcriptome library constructed from 12 adult specimens revealed 25 toxin or toxin-like genes. Transcripts were validated (RT-qPCR) by assessing the differential expression of the toxin-like PpenTox1 transcript and normalising with housekeeping genes.

Results: Analysis of the coxI-gene revealed a similarity to other species of the family Phrurolithidae. Transcriptome analysis also revealed similarity with venom components of species from the families Ctenidae, Lycosidae, and Sicariidae. Expression of the toxin-like PpenTox1 gene was different for each developmental stage (juvenile or adult) and also for both sexes (female or male). Additionally, a partial sequence was obtained for the toxin-like PpenTox1 from DNA.

Conclusion: Data from the amplification of the mitochondrial coxI gene confirmed that P. pennimani belongs to the family Phrurolithidae. New genes and transcripts coding for venom components were identified.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.80
自引率
8.30%
发文量
39
审稿时长
6-12 weeks
期刊介绍: Journal of Venomous Animals and Toxins including Tropical Diseases (JVATiTD) is a non-commercial academic open access publication dedicated to research on all aspects of toxinology, venomous animals and tropical diseases. Its interdisciplinary content includes original scientific articles covering research on toxins derived from animals, plants and microorganisms. Topics of interest include, but are not limited to:systematics and morphology of venomous animals;physiology, biochemistry, pharmacology and immunology of toxins;epidemiology, clinical aspects and treatment of envenoming by different animals, plants and microorganisms;development and evaluation of antivenoms and toxin-derivative products;epidemiology, clinical aspects and treatment of tropical diseases (caused by virus, bacteria, algae, fungi and parasites) including the neglected tropical diseases (NTDs) defined by the World Health Organization.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信