β -内酰胺类抗生素受体blar-ctd异源表达、纯化及分析系统应用过程中的生物活性监测

P. Semizhon, H.P. Scheslenok, I. V. Harbachova, I. Vashkevich, O. Sviridov
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引用次数: 0

摘要

为了发展食品中β -内酰胺类抗生素的生物检测技术,制备了高纯度的β -内酰胺受体BlaR-CTD并对其进行了表征。阐述了一种从异源性表达、分离和试剂制备过程中定量测定生物活性和免疫反应性受体的测试系统。研究了BlaR-CTD的免疫化学性质和抗生素结合能力,提出了利用微孔板或色谱试纸条检测β -内酰胺类抗生素的受体免疫系统的构建和应用方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MONITORING OF BIOLOGICAL ACTIVITY OF BETA-LACTAM ANTIBIOTICS RECEPTOR BLAR-CTD IN THE PROCESSES OF ITS HETEROLOGICAL EXPRESSION, PURIFICATION AND APPLICATION IN ANALYTICAL SYSTEMS
To develop the technologies of beta-lactam antibiotics bioassays in foods, high purity beta-lactam receptor BlaR-CTD has been obtained and characterized. A test system for the quantitation of the biologically active and immunoreactive receptor in the processes of its heterological expression, isolation and reagent forms preparation was elaborated. The immunochemical properties and antibiotic binding ability of BlaR-CTD were investigated, and the constructions and application procedures of receptor-immune systems for the assay of beta-lactam antibiotics using microplates or chromatographic test-strips were proposed.
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